In Noll’s experiment , explain where DNase I cuts the DNA. Why were the bands on the gel in multiples of 200 bp at lower DNase I concentrations? TESTING THE HYPOTHESISFIGURE I0.11 DNe I ctschromatin o repeatina unts contaning 30 bpof DNA.Starting mater ial: Nuclei from rat liver ells.Experimental levdCanaptud levd1. Incubute the nuclei with low, medium,and high concentrations of DNase I.The conceptual level illustrates a lowDNase I conentration.DNase IBefore digestion(beads onLowMediumHigha string)37°C37°C37°CA fter digestion(DNA is cut ininker region)Treat with delergent; add phenol.2 lolale the DNA. This involvesdicsolving the nuck ar membrane withdelergent and treating the sample withthe organic solvent phenol.Аquеоияphase(containsDNA)DNA inPhenolphae (containsmembranes andsnoonbephaseprokeima)Marker LowMedium HighLow3. Load the DNA into a well of an agarusegel and run the gel to separale the DNApieces according to sie. On this el,also load DNA fragments of knownmolecular mas (marker lane).సి గాGel (top view)Stain el.4. Visualze the DNA fragments bystaining the DNA with ethidumbromide, a dye that binds to DNA andis fluorescent when exciled by UV lightSolutionGelwithethidiumbromideView gelUV lightPhotograph gel.

Name: In Noll’s experiment , explain where DNase I cuts the DNA. Why were th
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Description: In Noll’s experiment , explain where DNase I cuts the DNA. Why were the bands on the gel in multiples of 200 bp at lower DNase I concentrations? TESTING THE HYPOTHESISFIGURE I0.11 DNe I ctschromatin o repeatina unts contaning 30 bpof DNA.Starting mat