A piece of DNA fragment is sequenced. cloned DNA fragment, and set up a series of four dideoxy reaction. You then separate the products of the reaction by gel electrophoresis and obtain the following banding patter: ddATP ddTTP ddCTP ddGTP
A piece of DNA fragment is sequenced. cloned DNA fragment, and set up a series of four dideoxy reaction. You then separate the products of the reaction by gel electrophoresis and obtain the following banding patter: ddATP ddTTP ddCTP ddGTP
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:A piece of DNA fragment is sequenced. You clone the the fragment, isolate the
cloned DNA fragment, and set up a series of four dideoxy reaction. You then
separate the products of the reaction by gel electrophoresis and obtain the
following banding patter:
ddATP
ddTTP
O 5'-ATTCGACT-3'
O 5'-TCAGCTTA-3'
What is the base sequence of the synthesized fragment?
O 5'-AGTCGAAT-3'
ddCTP
O 5'-TAAGCTGA-3'
I
ddGTP
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