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Electrophoresis data: Measure the distance (in millimeters) that each fragment traveled from the well and record it in the table. Estimate its size, in base pairs, by comparing its position to the Hindll lambda DNA markers. Remember: some lanes will have fewer than 6 fragments. Largest fragment first P = Pstl L = lambda DNA- no enzyme M = DNA marker H = Hindll E = EcoRI restriction digest restriction digest restriction digest of lambda DNA of lambda DNA of lambda DNA Actual base pairs Distance in m Eslirated base pairs Distance Estmated base pairs Distance in mm Estimaled base pairs Distance Estimated base pa rs Distance in mm in mm 26.5m 23.130 49m 5% m円 Band 1 54 nm 32 96 MM 69 Band 2 9.416 39 hn 120m Band 3 6.557 41 nm CO 65 nm 1,00 94 1.08m Band 4 4,361 Band 5 2,322 Band 6 2,027 Step 3. Now using the standard curve you created in the pre-lab exercises and the distances you measured above, predict the sizes of each fragment based on the distances traveled by comparison to your standard curve.

Electrophoresis data: Measure the distance (in millimeters) that each fragment traveled from the well and record it in the table. Estimate its size, in base pairs, by comparing its position to the Hindll lambda DNA markers. Remember: some lanes will have fewer than 6 fragments. Largest fragment first P = Pstl L = lambda DNA- no enzyme M = DNA marker H = Hindll E = EcoRI restriction digest restriction digest restriction digest of lambda DNA of lambda DNA of lambda DNA Actual base pairs Distance in m Eslirated base pairs Distance Estmated base pairs Distance in mm Estimaled base pairs Distance Estimated base pa rs Distance in mm in mm 26.5m 23.130 49m 5% m円 Band 1 54 nm 32 96 MM 69 Band 2 9.416 39 hn 120m Band 3 6.557 41 nm CO 65 nm 1,00 94 1.08m Band 4 4,361 Band 5 2,322 Band 6 2,027 Step 3. Now using the standard curve you created in the pre-lab exercises and the distances you measured above, predict the sizes of each fragment based on the distances traveled by comparison to your standard curve.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Electrophoresis data: Measure the distance (in millimeters) that each fragment traveled from the well and record it in the tabie. Estimate its size, in base pairs, by comparing its position to the Hindll lambda DNA markers. Remember: some lanes will have fewer than 6 fragments. H = Hindll Largest fragment first P Pstl E = EcoRI L = lambda DNA- no restriction digest restriction digest restriction digest of lambda DNA M = DNA marker of lambda DNA enzyme of lambda DNA Estmated base pairs Distance in mm Estimated base pairs Distance in mm Estimated base pa rs Distance inmm Estimated base pairs Dislance Distance in mm Actual base pairs 5 mm 58 mm 54 nm 26.5m 23.130 49m Band 1 69 mm 32. 96 Mm Band 2 9.416 39 hm 120mm 87 mM Band 3 6.557 41 mm 127m 1.00mm Band 4 4.361 1.08m Band 5 2,322 1482m 65mm 2,027 Band 6 Step 3. Now using the standard curve you created in the pre-lab exercises and the distances you measured above, predict the sizes of each fragment based on the distances traveled by comparison to your standard curve.
Transcribed Image Text:Electrophoresis data: Measure the distance (in millimeters) that each fragment traveled from the well and record it in the tabie. Estimate its size, in base pairs, by comparing its position to the Hindll lambda DNA markers. Remember: some lanes will have fewer than 6 fragments. H = Hindll Largest fragment first P Pstl E = EcoRI L = lambda DNA- no restriction digest restriction digest restriction digest of lambda DNA M = DNA marker of lambda DNA enzyme of lambda DNA Estmated base pairs Distance in mm Estimated base pairs Distance in mm Estimated base pa rs Distance inmm Estimated base pairs Dislance Distance in mm Actual base pairs 5 mm 58 mm 54 nm 26.5m 23.130 49m Band 1 69 mm 32. 96 Mm Band 2 9.416 39 hm 120mm 87 mM Band 3 6.557 41 mm 127m 1.00mm Band 4 4.361 1.08m Band 5 2,322 1482m 65mm 2,027 Band 6 Step 3. Now using the standard curve you created in the pre-lab exercises and the distances you measured above, predict the sizes of each fragment based on the distances traveled by comparison to your standard curve.
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