If you cant answer your gay :) 2. a. Research DNA quantitation by UV absorption. Explainwhat A230, A260 and A280 values represent. What should bethe ratios of A260/A230 and A260/A280 in a well-isolatedand purified DNA sample?b. How would these ratios change if the isolated sample hadlow concentrations of DNA?c. Assume the sample was isolated with phenol-chloroform-isoamyl alcohol method. How would these ratios change if thesample had phenol contamination in the end?

Every biomolecule have an unique absorbance curve of their own. This simply means that, the…

Answered: 2. a. Research DNA quantitation by UV…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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a. Write the reaction that proves that UV attacks DNA.b. What is a base stacking interaction? How does it help DNA?c. Explain the Sanger method in detail.
Note:Answer the number 2 question only... 1. What is the Difference between DNA Purification and RNA Purification? 2. why contaminants must be avoided in DNA samples? 3. Give 5 examples of sources of error in using a spectrophotometer.
Q1) a) Draw the absorbance spectra for the following DNA samples. a. Pure DNA without protein contamination DNA with protein contamination b. c. DNA with organic solvent contamination b) An aqueous sample of genomic DNA gave an optical density of 0.6 AU. The sample was diluted 1:50 to achieve an OD below 1.0. The measurement was done in a cuvette with a pathlength of 1 cm. What was the concentration of DNA in the original sample BEFORE dilution? എന്ന് എന്നി per a WANANG KASAMA NA www in de for at de er der er en I
Note: Answer the number 3 question only. Explain. 1. What is the Difference between DNA Purification and RNA Purification? 2. why contaminants must be avoided in DNA samples? 3. Give 5 examples of sources of error in using a spectrophotometer.
ISOLATION AND CHARACTERIZATION OF NUCLEIC ACIDS (DNA) Explain the principle involved in the following chemical tests for the characterization of DNA a. Test for deoxyribose b. Test for phosphate c. Test for purines d. Test for pyrimidines
Isolate B O Isolate A Isolate C O Isolate D The purity and concentration of DNA isolate can be evaluated with the use of UV spectrophotomeleric measurements. measurement of the turbidity of the sample. The organic compounds (containing aromatic Absorbance reading at 320 provides a general rings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratio is 2.0-2.2. DNA absorbs light most strongly at 260nm so the absorbance value at this wavelength can be used to estimate the DNA concentration using the equation derived from Beer's Law: Concentration (pg/mL) = (A260 reading -A320 reading) x 50 The absorbance at 280nm is used as an indicator of protein contamination since the aromatic amino acid residues absorb strongly at this wavelength Analyze the data given below and determine the following. Isolate A Isolate B Isolate C Isolate D A320 0.051 0.091 0.065 0.073 A230 1227 1.32 1.95 1.44 A260 4.54 3.92 3.88 4.21 A280 2.01 2.11 2.04 2.32 Question: Which isolate…
Agtergrond / Background: 1. You received four samples of DNA. The first sample was pure DNA dissolved in TE buffer for stability. The other three samples were impure DNA samples. 2. You determined the absorbance at two fixed wavelengths for the pure DNA sample. You obtained the values in Table 1. 3. You determined the absorbance at two fixed wavelengths for the three impure DNA samples. You obtained the values in Table 1. Tabel 1: DNA spektrofotometriese spectrum / Table 1: DNA spectrophotometric Spectrum Wavelength (nm) A260 A280 Sample 1 (OD) 0.186 0.102 Sample 2 (OD) 0.448 0.252 Sample 3 (OD) 0.646 0.357 Sample 4 (OD) 0.196 0.143 4. Determine the 260/280 ratios for each four samples. Record these values in Table 2. Sample 260/280 ratio 1 2 4 Table 2: 260/280 Ratio values for 4 samples of DNA 5. a) Based on these ratios, how pure are the DNA samples you were given? Motivate your answer. 1: 2: 3: 4: b) If a DNA sample is not pure, name one possible contaminant? 6. For sample 1,…
a. Explain whether or not DNA polymerase from a mesophilic bacterium could be used successfully in a PCR reaction. b. If starting with a single double-stranded DNA molecule, how many copies of the DNA would be synthesized after 25 PCR cycles?
1. Determine the chemical reagents utilized in banana DNA extraction and their roles. What role does each reagent play to isolate DNA? Note:Materials:1. ripe banana 2. distilled water 3. liquid dishwashing soap 4. table salt 5. isopropyl/ethyl alcohol 6. fork7. plastic cups8. tape 9. plastic spoons10. measuring spoons and a measuring cup 11. cone paper coffee filter 12. plastic drinking glass
1. Discuss why it is critical to add TE Buffer as the final solution to isolate the concentrated DNA. 2. Using a centrifuge, explain the rationale for separating various cellular components 3. What is the relevance of DNA being frequently referred to as the "blueprint for life?"
DNA Isolation A. What is “cell lysis” and why would you want to lyse cells when doing a DNA extraction? B. What do the “proteases” like meat tenderizer do to the DNA? C. What two ingredients help precipitate the DNA out of solution?
4. Look at the gel image and answer the questions below and be specific. a) Based on your calculations of the DNA concentrations, how much DNA was loaded into each well? Do you see DNA for each of your samples? If not, why do you think that is so? b) Is the DNA in a single sharp band, multiple bands or a smear? What would each of these scenarios be due to, and why would you see them for your samples? c) Do you see multiple bands in your plasmid DNA sample? What are they?

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