1.a. Match the plasmid illustration to its gel lane. 1.b What is the size of the longest DNA fragment in samples A. B. and C.?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A B C
kb
kb
Eco RI
Eco RI
3.0
1.1
kb
2.2
1.9
1.4
22
1.9
1.9
Eco RI
Bam HI Eco RI
Bam HI
1.2
1.5
kb
1.9
1.1
0.3
1.2
1.0
1.4
1.2
1.1
1.0
Bam HI
Bam HI
1.3
kb
0.3
3.0
1.5
1.0
1.a. Match the plasmid illustration to
its gel lane. 1.b What is the size
of the longest DNA fragment in
samples A. B. and C.?
2. How many fragments will be formed
after digestion in sample C, if the
plasmid is circular and why?
3. What are the critical steps in
transformation?
4. How was RAD52 expression
observed?
5. Provide current applications of DNA
Technology.
Transcribed Image Text:A B C kb kb Eco RI Eco RI 3.0 1.1 kb 2.2 1.9 1.4 22 1.9 1.9 Eco RI Bam HI Eco RI Bam HI 1.2 1.5 kb 1.9 1.1 0.3 1.2 1.0 1.4 1.2 1.1 1.0 Bam HI Bam HI 1.3 kb 0.3 3.0 1.5 1.0 1.a. Match the plasmid illustration to its gel lane. 1.b What is the size of the longest DNA fragment in samples A. B. and C.? 2. How many fragments will be formed after digestion in sample C, if the plasmid is circular and why? 3. What are the critical steps in transformation? 4. How was RAD52 expression observed? 5. Provide current applications of DNA Technology.
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