Spectrophotometric evaluation of the extracted nucleic acid can provide information on A. Quantity of DNA and quantity of RNA in the same sample B. Presence of plasmid DNA C. Presence of contaminants D. Quantitiy of nucleic acid E. Integrity of the nucleic acid
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- which sample shows the plasmid is fully digestedIn which reagent is extracted DNA suspended to put it in solution? O Sodium dodecyl sulfate (SDS) Sodium citrate saline buffer Cold absolute alcohol OH2SO4 O HCIAfter adding dna ligase to plasmid alone the sample could serve as: experimental sample negative control wasteof material positive controls
- Samples came to the laboratory for analysis. You are asked to identify the differences between these examples. It is known that the DNA of the samples must first be isolated in order to understand the difference. Isolated to DNA. It's time for the next step. It was decided to use the RAPD technique. The amount of DNA in the samples isolated in the spectrophotometer was determined. The sample is measured at 250 ng / microliter. In the RAPD technique, the total reaction volume for PCR is optimized to be 15 microliters. This is adjusted to contain 5 microliters of DNA in 15 microliters. This technique requires 25 ng of DNA to work. How to dilute the measured 250 ng / microliter sample DNA into the appropriate amount for the reaction to occur?Question Image hown bele nallest? Q. A gel from gel electrophoresis is shown below. Which DNA fragment is the smallest? B. Direction of TravelA description of the principles of agarose gel electrophoresis of DNA.
- Drag and drop the appropriate text in the gaps below. Firstly, the plasmid DNA in the E. coli is propagated through Then the bacterial cells are pelleted by centrifugation at 10,000 RPM. The media supernatant is removed and the bacterial cells are The bacterial cells are then lysed via |. Contaminating macromolecules such as protein and chromosomal DNA is removed by Overnight incubation of the bacterial culture washed in cell resuspension buffer addition of lysis buffer addition of neutralisation buffer washing of the spin column separation via agarose gel electrophoresisYou are provided with coiled DNA and plasmid DNA that you subject to gel electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. Exact fragment sizes are not important.Summary on how to use a spectrophotometer DNA nanodrop.
- The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily based on: O The number of the DNA fragments. The size of the DNA fragments. The concentration of the DNA NoneAdvantages and disadvantages of using nucleic acid detection assay in diagnostic virologyWhich statement about plasmid is FALSE: Plasmid is nonessential DNA in the bacteria Plasmids replicate independent of the genome replication. Their size can vary from a few kilobases to a few hundred kilobases. They are all circular.