Why is DNA quantification so important after the isolation of DNA? (1) Answer. 2. Give two mostly used methodology for quantifying the amount of nucleic acid in a preparation. (1) Answer. 3. How would you dilute a 3N HCl stock solution in a ratio of 1:10?

Q: ...is always equal A, A + G... to T+ C. G C Purines Pyrimidines %3D [T]+[C]+[G]=100% #C Source of…

A: The Chargaff's rule says that in DNA there is always equality in quantity between the bases A and T…

Q: why would an EndA+ strain potentially be an issue for DNA Purification if the optional wash step…

A: DNA purification is the process of isolating DNA from a biological sample by removing contaminants…

Q: If your DNA extraction were a concentration of 45ng DNA/ug, how would you prepare 500 ul of a 1…

A: The hereditary molecule deoxyribonucleic acid (DNA) transmits genetic information from one…

Q: Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and  70%…

A: 70% ethanol is used in washing DNA furing DNA extraction. 100% ethanol is used in the final step. In…

Q: To carry out sequencing, you need to include 600 ng of DNA and a total of 6.4 pmol of sequencing…

A: DNA, or deoxyribonucleic acid is a long double-stranded molecule that carries the genetic…

Q: Q1) a) Draw the absorbance spectra for the following DNA samples. a. Pure DNA without protein…

A: “Since you have posted multiple questions with multiple sub parts, we will provide the solution only…

Q: Scenario 1: DNA concentration? UV-Vis spectroscopy [Choose ] Homology Modeling Scenario 2: Does it…

A: Different techniques are used for determining different properties.

Q: 1. Why do we need to check the isolated DNA for its quantity and quality? 2. The purity of DNA…

A: Deoxyribonucleic acid DNA isolation is referred to the extraction process of DNA from different…

Q: Estimate the viscosith of 1.0 vol % agarose gel solution if it took 58 minutes for 1500 base pairs…

A: In order to solve this problem, we need to first calculate electrophoretic mobility () during the…

Q: Why was there a greater A260 absorbance reading for your DNA sample that was incubated at higher…

A: Hyperchromicity is the increase in optical density of a substance at a particular wavelength.

Q: You add 3.0 µg of plasmid DNA to a QIAprep spin miniprep purification column. You perform the…

A: Negative charge of silanol: A silica membrane is commonly seen in QIA prep spin mini prep…

Q: Could you please explain these:   1. Why analyze the unknowns in a (short) serial dilution rather…

A: Introduction: DNA concentration refers to the amount of DNA present in a sample. It is typically…

Q: In the equation describing Tm of DNA: Tm=81.6+0.41(GC %) + 16.6 log [C], the term 81.6 °C represents…

A: Polymerase Chain Reaction (PCR) is a molecular protocol allowing the production of a large number of…

Q: TYPE in the answers into the blank cells of the table, you will have to use the scroll bar to see…

A:

Q: What would spread (fragmented) bands in the electrophoresis gel tell you about the quality of your…

A: Gel electrophoresis is a technique used to separate DNA fragments according to their size. The DNA…

Q: 1) Prepare the following enzymatic reaction, present it in tabulated form. In a final volume of 30…

A: Enzymatic reactions are essential for all biological processes. They are typically much faster than…

Q: Explain why a higher agarose concentration is preferred when seperating smaller DNA molecules.…

A: The process of agarose gel electrophoresis is used to separate nucleic acids, primarily linear DNA,…