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A: Ans 1 : Point mutation
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- Give 1 example of diseases arising from each of the following mutations and explain each.
- Point mutation
- Frameshift mutation
- Calculate for the DNA concentration. The absorbance at 260 nm is 0.7 at 1:30 dilution; the absorbance at 320 nm is 0.1.
- Is the DNA isolated of good quality? The absorbance at 260 nm is 0.7; the absorbance at 280 nm is 0.1; the absorbance at 320 is 0.01.
- If you do an absorbance reading after plasmid purification and get an A260/A280 of less than 1.8, how could you further purify the sample to get rid of the protein contamination? Is it always necessary to have completely pure DNA? What are some cases where it would or would not be?
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- There are various types of DNA-targeting drug, including DNA alkylating agents, DNA intercalators and DNA chain terminators. SI Suggest why drugs targeting DNA can be useful for treating cancers and viral infections. Describe TWO structural features that are necessary or useful for drugs to act as DNA intercalators. Describe TWO structural features that are necessary or useful for drugs to act as DNA chain terminators. L V ElIn analyzing the base composition of a DNA sample, a student loses the information on pyrimidine content. The purine content is A = 27% and G = 23%. Using Chargaffs rule, reconstruct the missing data and list the base composition of the DNA sample.Define and compare the following types of nucleotide substitutions. Which is likely to cause the most dramatic mutant effect? a. missense mutation b. nonsense mutation c. sense mutation
- A certain section of the coding (sense) strand of some DNA looks like this: 5'- ATGGGCCACTCATCTTAG-3' It's known that a very small gene is contained in this section. Classify each of the possible mutations of this DNA shown in the table below. I Don't Know mutant DNA 5'- ATG GGCCACAGTTCTTAG-3' 5'- ATG GG CTCATCTTAG - 3' 5'- ATG GGCCACGCATCTTAG-3' Submit type of mutation (check all that apply) ооооо O point O silent O noisy ооооо insertion deletion insertion O deletion Opoint Osilent noisy insertion O deletion ооооо Opoint silent O noisy X S Ⓒ2023 McGraw Hill LLC. All Rights Reserved. Terms of Use | Privacy Center AccessibilityWhy is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…What will be the Tm of DNA that has cytosine plus guanine content of 30% compared to that of DNA that has a cytosine plus guanine content of 50% if both are heated under the same experimental conditions? lower higher same impossible to determine from information given
- Table I CACGT A GA CTGAGG ACTC CACGTAGACTGAG G ACAC Wild-type beta-globin gene fragment Sickle-cell beta-globin gene fragment > Circle the mutation in DNA of the sickle-cell beta-globin gene fragment Compare fragments of DNA the wild-type and mutant beta-globin genes in the Table I above, what are the similarities and differences you observe?In order to determine the purity of a DNA sample. spectrophotometry can be carried out at calculated, and a number between to measure DNA, and to measure protein. The ratio is then indicates higher purity. 280 nm; 260 nm; A280/A260; 0.5-1 260 nm; 280 nm; A260/A280; 0.5-1 260 nm; 280 nm; A280/A260; 1.5-2 260 nm; 280 nm; A260/A280; 1.5-216/ The phosphates that make up the phosphodiester bonds in DNA have pKa 2. What is the sign of the charge on the DNA when the pH of solution is neutral? Postive Negative Neutral Not enough information given 17/ The phosphates that make up the phosphodiester bonds in DNA have pKa 2. What fraction of the phosphates in DNA are charged at neutral pH? essentially all of them about half of them about 1/4 of them none of them Not enough information given 18/ The phosphates that make up the phosphodiester bonds in DNA have pKa 2. What is the charge of Mycoplasma genitalium DNA, which is 586-kilo-base-pairs (kbp) long, at neutral pH?
- DNA Polymerase Holoenzyme IIlI is represented below. It consists of several domains with distinct functions. The structure capable of 5' to 3' DNA polymerization is represented by the letter and the structure capable of 3' to 5' exonuclease activity is represented by the letter B. -D DNA Pol II holoenzyme D; A А; С А;B А А; D В; D O OThe temperature at which a DNA sample denatures can be used to estimate the proportion of its nucleotide pairsthat are G- C. What would be the basis for this determination, and what would a high denaturation temperature for aDNA sample indicate?Isolate B O Isolate A Isolate C O Isolate D The purity and concentration of DNA isolate can be evaluated with the use of UV spectrophotomeleric measurements. measurement of the turbidity of the sample. The organic compounds (containing aromatic Absorbance reading at 320 provides a general rings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratio is 2.0-2.2. DNA absorbs light most strongly at 260nm so the absorbance value at this wavelength can be used to estimate the DNA concentration using the equation derived from Beer's Law: Concentration (pg/mL) = (A260 reading -A320 reading) x 50 The absorbance at 280nm is used as an indicator of protein contamination since the aromatic amino acid residues absorb strongly at this wavelength Analyze the data given below and determine the following. Isolate A Isolate B Isolate C Isolate D A320 0.051 0.091 0.065 0.073 A230 1227 1.32 1.95 1.44 A260 4.54 3.92 3.88 4.21 A280 2.01 2.11 2.04 2.32 Question: Which isolate…