) *When double-stranded DNA is heated, the two strands separate into single strands in a process referred to as "denaturation" or "melting" (different meaning than the physical process of a phase transition from solid to liquid). The temperature at which half of the duplex DNA molecules are intact and half have melted is defined as the melting temperature, often abbreviated as Tm. Under standard conditions, the expected melting temperature in degrees Celsius can be calculated from the equation: Tm = 59.9 + [0.41 x %(G+C)] – (675/length of DNA duplex); %3D Note: %(G+C) represents the fraction of the total base pairs that are G-C or C-G, expressed as a whole number percentage (e.g. if two-fifths of the base pairs were G-C or C-G, then %(G+C) would be 40). a) Does the Tm increase or decrease if there are more G+C (and thus fewer A+T) base pairs? Why? b) Does the Tm increase or decrease as the length of DNA increases? Why? c) Calculate the predicted Tm for a stretch of double helix that is 100 nucleotides long and contains 50% G+C content.

) *When double-stranded DNA is heated, the two strands separate into single strands in a process referred to as "denaturation" or "melting" (different meaning than the physical process of a phase transition from solid to liquid). The temperature at which half of the duplex DNA molecules are intact and half have melted is defined as the melting temperature, often abbreviated as Tm. Under standard conditions, the expected melting temperature in degrees Celsius can be calculated from the equation: Tm = 59.9 + [0.41 x %(G+C)] – (675/length of DNA duplex); %3D Note: %(G+C) represents the fraction of the total base pairs that are G-C or C-G, expressed as a whole number percentage (e.g. if two-fifths of the base pairs were G-C or C-G, then %(G+C) would be 40). a) Does the Tm increase or decrease if there are more G+C (and thus fewer A+T) base pairs? Why? b) Does the Tm increase or decrease as the length of DNA increases? Why? c) Calculate the predicted Tm for a stretch of double helix that is 100 nucleotides long and contains 50% G+C content.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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