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Isolate B Isolate A Isolate C Isolate D The purity and concentration of DNA isolate can be evaluated with the use of UV spectrophotomelenc measurements. measurement of the turbidity of the sample. The organic compounds (containing aromatic Absorbance reading at 320 provides a general hings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratio is 2.0-2.2. DNA absorbs light most strongly at 260nm so the absorbance value at this wavelength can be used to estimate the DNA concentration using the equation derived from Beer's Law Concentration (pg/mL) = (A260 reading-A320 reading) x 50 The absorbance at 280nm is used as an indicator of protein contamination since the aromatic amino acid residues absorb strongly at this wavelength Analyze the data given below and determine the following Isolate A Isolate B Isolate C Isolate D A320 0.051 0.091 0.065 0.073 A230 1.95 144 A260 4.54 3.92 3.88 4.21 A280 201 2.11 2.04 232 Question: Which isolate has the ideal 260/230 ratio?

Isolate B Isolate A Isolate C Isolate D The purity and concentration of DNA isolate can be evaluated with the use of UV spectrophotomelenc measurements. measurement of the turbidity of the sample. The organic compounds (containing aromatic Absorbance reading at 320 provides a general hings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratio is 2.0-2.2. DNA absorbs light most strongly at 260nm so the absorbance value at this wavelength can be used to estimate the DNA concentration using the equation derived from Beer's Law Concentration (pg/mL) = (A260 reading-A320 reading) x 50 The absorbance at 280nm is used as an indicator of protein contamination since the aromatic amino acid residues absorb strongly at this wavelength Analyze the data given below and determine the following Isolate A Isolate B Isolate C Isolate D A320 0.051 0.091 0.065 0.073 A230 1.95 144 A260 4.54 3.92 3.88 4.21 A280 201 2.11 2.04 232 Question: Which isolate has the ideal 260/230 ratio?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Isolate B O Isolate A Isolate C O Isolate D The purity and concentration of DNA isolate can be evaluated with the use of UV spectrophotomeleric measurements. measurement of the turbidity of the sample. The organic compounds (containing aromatic Absorbance reading at 320 provides a general rings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratio is 2.0-2.2. DNA absorbs light most strongly at 260nm so the absorbance value at this wavelength can be used to estimate the DNA concentration using the equation derived from Beer's Law: Concentration (pg/mL) = (A260 reading -A320 reading) x 50 The absorbance at 280nm is used as an indicator of protein contamination since the aromatic amino acid residues absorb strongly at this wavelength Analyze the data given below and determine the following. Isolate A Isolate B Isolate C Isolate D A320 0.051 0.091 0.065 0.073 A230 1227 1.32 1.95 1.44 A260 4.54 3.92 3.88 4.21 A280 2.01 2.11 2.04 2.32 Question: Which isolate has the ideal 260/230 ratio?
Transcribed Image Text:Isolate B O Isolate A Isolate C O Isolate D The purity and concentration of DNA isolate can be evaluated with the use of UV spectrophotomeleric measurements. measurement of the turbidity of the sample. The organic compounds (containing aromatic Absorbance reading at 320 provides a general rings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratio is 2.0-2.2. DNA absorbs light most strongly at 260nm so the absorbance value at this wavelength can be used to estimate the DNA concentration using the equation derived from Beer's Law: Concentration (pg/mL) = (A260 reading -A320 reading) x 50 The absorbance at 280nm is used as an indicator of protein contamination since the aromatic amino acid residues absorb strongly at this wavelength Analyze the data given below and determine the following. Isolate A Isolate B Isolate C Isolate D A320 0.051 0.091 0.065 0.073 A230 1227 1.32 1.95 1.44 A260 4.54 3.92 3.88 4.21 A280 2.01 2.11 2.04 2.32 Question: Which isolate has the ideal 260/230 ratio?
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