Assume that the extinction coefficient for DNA at 260 nm is 20 g-1 x L x cm-1. What would be the DNAconcentration of a solution if its 25x dilution has an absorbance at 260 nm of 0.6?

Extinction coefficient, sometimes called "extinction coefficient" in meteorology or climatology,…

Answered: Assume that the extinction coefficient…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Suppose that you are tasked to determine the protein concentration of an unknown protein solution via Bradford assay. You prepared six solutions of bovine serum albumin (BSA) with different concentrations. The initial concentration of the BSA stock solution is 7.50 mg/mL. Approximately 200 µL of Bradford Reagent was added to each of these solutions and the absorbance at 595 nm was taken after 5 minutes. See the table below for data on the standard solutions. Standard # A595 BSA conc (mg/mL) 0.000 0.158 2 1.125 0.291 2.250 0.372 4 3.375 0.503 5 4.500 6 5.625 0.675 Determine the protein concentration, in mg/mL, of the unknown solution if its absorbance at 595 nm was 0.248. Note: Final answer format must be x.xx (two decimal places). Round off only in the final answer. Do not round off in the middle of calculation.
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Assume that the extinction coefficient for DNA at 260 nm is 20 g-1 x L x cm-1. What would be the D concentration of a solution if its 25x dilution has an absorbance at 260 nm of 0.6?