(ii)(iii)(c) To conduct a successful Enhanced Biological Phosphorus Removal process, aquick-shifting betweenandconditions are required to forcephosphorus-accumulating microbes to store more PolyP than they normally do. 2. Answer the following questions (a) - (c)(a) What are the five necessary components to conduct a successful PCR reaction?(1)(ii)(iv)(v)MgCl2 and Reaction Buffer(b) Briefly explain the purposes of the three steps involved in a PCR cycle.

The PCR reaction exponentially increases the target DNA area by going through the annealing,…

Answered: 2. Answer the following questions (a) -…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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5. Watch the video: https://www.youtube.com/watch?v=ml0Fo9kaWqo&t=38s. Based on the information in this video and material covered in class answer the following questions: A. In a Sanger sequencing-based PCR reaction, how many primers are added? B. In a Sanger sequencing-based PCR reaction, which molecules are more common: DNTPS or fluorescent terminators? C. What is a typical length of sequence that can be acquired using traditional (Sanger) sequencing? D. The results from sequencing one stretch of DNA is called a "read". How many reads can be obtained from a run on a 384-well plate Sanger sequencing instrument vs. a NovaSeg instrument? E. How many gjgabases of sequences can be obtained on the Sanger vs. NovaSea instruments? Regarding F. and G., the adaptor sequences have two parts: primer binding sites and capture sequences. F. Once the (denatured) DNA has been loaded into the flow cell, which adaptor sequence part is hybridized to other single-stranded DNA first? G. How many copies…
28. In a transformation experiment, DNA is collected from an E. coli donor strain of genotype cys(+) leu(-) thr(+) and used to transform a recipient of genotype cys(-) leu(+) thr(-). Initially, the treated recipient population is plated on a minimal medium supplemented with threonine. Many colonies are obtained. What are possible genotypes of these colonies? A. cys(-) leu(+) thr(+) B. cys(+) leu(+) thr(-) C. All are cys(+) and either + or - for leu and thr D. All are thr(+) and either + or - for cys and leu E. All are thr(-) cys(-) and either + or - - for CYS
The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis ofshort tandem repeats, STRs, in the human genome. This approach has many distinct advantages.Please list and explain three of those advantages.
Droplet based single cell DNA sequencing uses a microfluidic platform which generates droplets encapsulating individual cells. A micrograph of such a device [Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are 25 μm deep. (a) (b) Oil Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm. Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show individual cells, and black arrows indicate flow direction. (c) RT/lysis reagents Cells Barcoding hydrogel beads A company wants to explore the design of a prototype of the device made from PDMS. Describe a suitable fabrication process, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. Testing of the prototype was successful and the company wants to explore the feasibility of manufacturing the device from glass. Describe the fabrication process for this…
1. Primers come at a concentration of 200 pmol//ul (uM). You want the final concentration of your primers to be 0.2 uM. How would you dilute the primers in water so that you can add 1 ul in a PCR mix with a total volume of 50ul?
1) Describe the ingredients of a PCR reaction. List different temperatures used in each cycle and tell why each is used.
With regard to DNA microarrays, answer the followingquestions:A. What is attached to the slide? Be specific about the numberof spots, the lengths of DNA fragments, and the origin ofthe DNA fragments.B. What is hybridized to the microarray?C. How is hybridization detected?
1.) What are the different temperatures used in PCR (polymerase chain reaction)? What happens at each temperature? 2.) What ingredients are used in PCR? What role does each ingredient have in replicating DNA? 3.) Why is the contamination of foreign DNA a particularly important problem when you do PCR?

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2. Answer the following questions (a) - (c) (a) What are the five necessary components to conduct a successful PCR reaction? (i) (ii) (111) (iv) (v) MgCl₂ and Reaction Buffer (b) Briefly explain the purposes of the three steps involved in a PCR cycle.