(ii)(iii)(c) To conduct a successful Enhanced Biological Phosphorus Removal process, aquick-shifting betweenandconditions are required to forcephosphorus-accumulating microbes to store more PolyP than they normally do. 2. Answer the following questions (a) - (c)(a) What are the five necessary components to conduct a successful PCR reaction?(1)(ii)(iv)(v)MgCl2 and Reaction Buffer(b) Briefly explain the purposes of the three steps involved in a PCR cycle.

The PCR reaction exponentially increases the target DNA area by going through the annealing,…

Answered: 2. Answer the following questions (a) -…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis ofshort tandem repeats, STRs, in the human genome. This approach has many distinct advantages.Please list and explain three of those advantages.
36. Which is the sequence of steps used to amplify DNA with PCR during a single cycle? 1) Primers bind (anneal) to complementary sequences of the target DNA. 2) DNA is heated to a high temperature to cause denaturation (DNA is single-stranded). 3) DNA polymerase extends the 3’OH of primers to make copies of the target DNA. A) 1, 2 and 3 B) 3, 2, and 1 C) 2, 1, and 3 D) 1, 3, and 2
Droplet based single cell DNA sequencing uses a microfluidic platform which generates droplets encapsulating individual cells. A micrograph of such a device [Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are 25 μm deep. (a) (b) Oil Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm. Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show individual cells, and black arrows indicate flow direction. (c) RT/lysis reagents Cells Barcoding hydrogel beads A company wants to explore the design of a prototype of the device made from PDMS. Describe a suitable fabrication process, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. Testing of the prototype was successful and the company wants to explore the feasibility of manufacturing the device from glass. Describe the fabrication process for this…
1. Primers come at a concentration of 200 pmol//ul (uM). You want the final concentration of your primers to be 0.2 uM. How would you dilute the primers in water so that you can add 1 ul in a PCR mix with a total volume of 50ul?
With regard to DNA microarrays, answer the followingquestions:A. What is attached to the slide? Be specific about the numberof spots, the lengths of DNA fragments, and the origin ofthe DNA fragments.B. What is hybridized to the microarray?C. How is hybridization detected?
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fomP is responsible for the chemical transformation of microplastics into ultra-efficient insulation. You take an arctic seawater sample and extract the DNA. 1. First you need to locate the gene on the bacterial chromosome. What procedure(s) would you use to identify and locate the gene? Explain how it/theywork(s). 2. Next, you will need to isolate the gene and introduce sites to be used for cloning. What would you use to make many copies of this gene? What will you need? How does it work on a molecular level?
Draw one cycle of PCR reaction below the following diagram
Hello, i understand the question, but i only need to know which option is right, no need for explanation. thanks in advance.
1.) What are the different temperatures used in PCR (polymerase chain reaction)? What happens at each temperature? 2.) What ingredients are used in PCR? What role does each ingredient have in replicating DNA? 3.) Why is the contamination of foreign DNA a particularly important problem when you do PCR?
PCR is a molecular biology technique where template DNA is amplified using a primer and oligonucleotides. The reaction is catalyzed by a thermostable DNA polymerase and in a particular reaction, the template strands are denatured at 95˚C. For strand hybridization, the melting temperature is 55˚C. What do you predict about the average duration of H bonds at the high temperature in comparison to the low temperature?

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2. Answer the following questions (a) - (c) (a) What are the five necessary components to conduct a successful PCR reaction? (i) (ii) (111) (iv) (v) MgCl₂ and Reaction Buffer (b) Briefly explain the purposes of the three steps involved in a PCR cycle.