In a Sanger sequencing-based PCR reaction, which molecules are more common:

Human Anatomy & Physiology (11th Edition)
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5. Watch the video: https://www.youtube.com/watch?v=ml0Fo9kaWqo&t=38s. Based on
the information in this video and material covered in class answer the following questions:
A. In a Sanger sequencing-based PCR reaction, how many primers are added?
B. In a Sanger sequencing-based PCR reaction, which molecules are more common:
DNTPS or fluorescent terminators?
C. What is a typical length of sequence that can be acquired using traditional (Sanger)
sequencing?
D. The results from sequencing one stretch of DNA is called a "read". How many
reads can be obtained from a run on a 384-well plate Sanger sequencing
instrument vs. a NovaSeg instrument?
E. How many gjgabases of sequences can be obtained on the Sanger vs. NovaSea
instruments?
Regarding F. and G., the adaptor sequences have two parts: primer binding sites and
capture sequences.
F. Once the (denatured) DNA has been loaded into the flow cell, which adaptor
sequence part is hybridized to other single-stranded DNA first?
G. How many copies of the original ssDNA are found in a cluster by the time the
sequencing primer is added?
H. The adaptor sequences have two parts: primer binding sites and capture
sequences.
I. Once the (denatured) DNA has been loaded into the flow cell, which adaptor
sequence part is hybridized to other single-stranded DNA first?
J. How many copies of the original ssDNA are found in a cluster by the time the
sequencing primer is added?
Transcribed Image Text:5. Watch the video: https://www.youtube.com/watch?v=ml0Fo9kaWqo&t=38s. Based on the information in this video and material covered in class answer the following questions: A. In a Sanger sequencing-based PCR reaction, how many primers are added? B. In a Sanger sequencing-based PCR reaction, which molecules are more common: DNTPS or fluorescent terminators? C. What is a typical length of sequence that can be acquired using traditional (Sanger) sequencing? D. The results from sequencing one stretch of DNA is called a "read". How many reads can be obtained from a run on a 384-well plate Sanger sequencing instrument vs. a NovaSeg instrument? E. How many gjgabases of sequences can be obtained on the Sanger vs. NovaSea instruments? Regarding F. and G., the adaptor sequences have two parts: primer binding sites and capture sequences. F. Once the (denatured) DNA has been loaded into the flow cell, which adaptor sequence part is hybridized to other single-stranded DNA first? G. How many copies of the original ssDNA are found in a cluster by the time the sequencing primer is added? H. The adaptor sequences have two parts: primer binding sites and capture sequences. I. Once the (denatured) DNA has been loaded into the flow cell, which adaptor sequence part is hybridized to other single-stranded DNA first? J. How many copies of the original ssDNA are found in a cluster by the time the sequencing primer is added?
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