What is shotgun sequencing and what technological advance was required to make this happen?
INTRODUCTION:- Shotgun sequencing involves randomly breaking up DNA sequences into lots of small pieces and then reassembling the sequence by looking for regions overlap'
-This method was used by fred sanger and his colleagues to sequence small genomes such as viruses and bacteria.
-In whole genome shotgun sequencing the entire genome is broken up into small fragments of DNA for sequencing.
-These fragments are often of varying sizes, ranging from 2-20 kilobases to 200-300 kb.
-These fragments are sequenced to determine the order of the DNA bases, A,C,G and T.
-The sequenced fragments are then assembled together by computer programs that find where fragments overlap.
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