Concepts of Biology
1st Edition
ISBN: 9781938168116
Author: Samantha Fowler, Rebecca Roush, James Wise
Publisher: OpenStax College
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Chapter 10, Problem 7RQ
What is the most challenging issue facing genome sequencing?
a. the inability to develop fast and accurate sequencing techniques
b. the ethics of using information from genomes at the individual level
c. the availability and stability of DNA
d. all of the above
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 10 Solutions
Concepts of Biology
Ch. 10 - Figure 10.7 Why was Dolly a Finn-Dorset and not a...Ch. 10 - n gel electrophoresis of DNA, the different bands...Ch. 10 - In the reproductive cloning of an animal, the...Ch. 10 - What carries a gene from one organism into a...Ch. 10 - What is a genetically modified organism (GMO)? a....Ch. 10 - What is the role of Agrobacterium tumefaciens in...Ch. 10 - What is the most challenging issue facing genome...Ch. 10 - Genomics can be used in agriculture to: a....Ch. 10 - What kind of diseases are studied using...Ch. 10 - What is the purpose and benefit of the polymerase...
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