Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
1. Compute the concentration of the standard solutions by completing Table B.1. Report your answers in 3 decimal places with proper unit. Show one representative calculation only. 2. Report the exact values of the linearity constant, slope, and y-intercept of the standard curve. 3. Calculate the dilution factor that you will use to calculate for the actual protein concentration. 4. Calculate the actual protein concentration in the sample (in mg/mL).

1. Compute the concentration of the standard solutions by completing Table B.1. Report your answers in 3 decimal places with proper unit. Show one representative calculation only. 2. Report the exact values of the linearity constant, slope, and y-intercept of the standard curve. 3. Calculate the dilution factor that you will use to calculate for the actual protein concentration. 4. Calculate the actual protein concentration in the sample (in mg/mL).

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
1. Compute the concentration of the standard solutions by completing Table B.1. Report your answers in 3 decimal places with proper unit. Show one representative calculation only. 2. Report the exact values of the linearity constant, slope, and y-intercept of the standard curve. 3. Calculate the dilution factor that you will use to calculate for the actual protein concentration. 4. Calculate the actual protein concentration in the sample (in mg/mL).
Transcribed Image Text:1. Compute the concentration of the standard solutions by completing Table B.1. Report your answers in 3 decimal places with proper unit. Show one representative calculation only. 2. Report the exact values of the linearity constant, slope, and y-intercept of the standard curve. 3. Calculate the dilution factor that you will use to calculate for the actual protein concentration. 4. Calculate the actual protein concentration in the sample (in mg/mL).
2. The seed extract of Salvia hispanica contains high concentration of protein. In order to quantify the protein content of Salvia hispanica, 2.5 grams of crude sample was dissolved in 5 mL of tris-HCI buffer. Two more dilutions of the crude sample were made as shown in Table B.2. It was subjected then to Bradford assay to determine its concentration against an external calibration standard as shown in Table B.1. Three-mL each of bovine serum albumin (BSA) standard solutions were prepared from a stock solution with concentration of 20 mg/mL. The relevant information is shown below: Table B.1. Absorbance readings of the BSA standard solutions. Initial volume of BSA stock solution, mL 0.00 0.15 0.30 0.45 0.60 0.75 Concentration, mg/mL (1) (2) (3) (4) (5) (6) 1 in 2 dilution 1 in 5 dilution Absorbance at 540 nm Table B.2. Absorbance readings of the crude extract: Sample Undiluted 0.000 0.124 0.201 0.298 0.363 0.434 Absorbance at 540 nm 0.548 0.438 0.244
Transcribed Image Text:2. The seed extract of Salvia hispanica contains high concentration of protein. In order to quantify the protein content of Salvia hispanica, 2.5 grams of crude sample was dissolved in 5 mL of tris-HCI buffer. Two more dilutions of the crude sample were made as shown in Table B.2. It was subjected then to Bradford assay to determine its concentration against an external calibration standard as shown in Table B.1. Three-mL each of bovine serum albumin (BSA) standard solutions were prepared from a stock solution with concentration of 20 mg/mL. The relevant information is shown below: Table B.1. Absorbance readings of the BSA standard solutions. Initial volume of BSA stock solution, mL 0.00 0.15 0.30 0.45 0.60 0.75 Concentration, mg/mL (1) (2) (3) (4) (5) (6) 1 in 2 dilution 1 in 5 dilution Absorbance at 540 nm Table B.2. Absorbance readings of the crude extract: Sample Undiluted 0.000 0.124 0.201 0.298 0.363 0.434 Absorbance at 540 nm 0.548 0.438 0.244
Expert Solution
steps

Step by step

Solved in 3 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS