1. Compute the concentration of the standard solutions by completing Table B.1. Report your answersin 3 decimal places with proper unit. Show one representative calculation only.2. Report the exact values of the linearity constant, slope, and y-intercept of the standard curve.3. Calculate the dilution factor that you will use to calculate for the actual protein concentration.4. Calculate the actual protein concentration in the sample (in mg/mL). 2. The seed extract of Salvia hispanica contains high concentration of protein. In order to quantify theprotein content of Salvia hispanica, 2.5 grams of crude sample was dissolved in 5 mL of tris-HCI buffer.Two more dilutions of the crude sample were made as shown in Table B.2. It was subjected then toBradford assay to determine its concentration against an external calibration standard as shown inTable B.1. Three-mL each of bovine serum albumin (BSA) standard solutions were prepared from astock solution with concentration of 20 mg/mL. The relevant information is shown below:Table B.1. Absorbance readings of the BSA standard solutions.Initial volume of BSA stocksolution, mL0.000.150.300.450.600.75Concentration,mg/mL(1)(2)(3)(4)(5)(6)1 in 2 dilution1 in 5 dilutionAbsorbance at540 nmTable B.2. Absorbance readings of the crude extract:SampleUndiluted0.0000.1240.2010.2980.3630.434Absorbance at 540 nm0.5480.4380.244

Bradford assay is a method to estimate the protein concentration in the given sample. It is based on…

Answered: 1. Compute the concentration of the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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6. GENERALIZATION Direction Arrange the procedure given below in order in preparing a solution. Use the box on the right. Write your answer on a separate sheet of paper. 1. Preparation of a Standard Solution The solid is dissolved in the water (1) by gently swirling the flask (with the stopper in place). A weighed amount of a substance (the solute) is put into the volumetric flask. More water is added, until the level of the solution reaches the (2) mark etched on the flask A small quantity of water is added to the solute. (3) (4)
Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.197, taking into account the dilution factor 100 per and total volume of extract 140ml
Purpose: what is the concentration of the menown Solution? Hypothesis: None. Method: Use the transport Activity - dry run to get the method Independent Variable Dependent Variable (measuring) (changing) Different metafities of sucrose Procedure: "as given" Data Table: Molarity in bag 0 (H₂0) 0.2 M Sucrose 0.4 M Sucrose 0.6 M Sucrose 0.8 M Sucrose 1.0 M Sucrose Unknown 90 38 40 initial mass (g) 20 10 10 10 10 Calculated channe final mass (g) 10.3 11.2 14.8 10.5 17.2 12.8 .2 Controlled Variables (same) Dialysis tubing, + time. (final mass-initial mass) initial mass .4 X 100 water % change my group Analysis 1. Calculate the percent change in mass for your solutions. Use the workspace in the data table for help. 2. Graph your data & the class average data. Label the independent (x axis) and dependent variable (y axis). Include a title. After plotting both sets of data one best fit STRAIGHT line. 100 Control The tube filled with water D % change class average 4 .8 46 68 30 1.0
A technician at a clinical laboratory received a blood serum sample for cholesterol analysis. In order to check the reliability of the procedure, the technician repeated the assay five times and obtained the results below. Use a spreadsheet statistical analysis to estimate the mean, standard deviation and standard error of the mean (SEM) for the data. Total cholesterol in blood serum sample in mg/100 mL (mg%): 157,154, 155, 152, 155. 2.
Please answer this question.
Answer the following problem using Reducing and Enlarging Formula (Factor Method)
Using 25 uL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ______ ul
2. For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. How much of the diluent must be added to tube 2 if 200uL Red cell suspension is added? Show pertinent solutions. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions.
Answer 1 and 2 with complete solutions
1. Study the given drug label and answer the question that follows. Show your solution How many total milliliters of diluent are used? What is the final concentration for each drug? a. b. C. How many milliliters are administered to give amoxicillin 500 mg/clavulanate 125 mg? J 343598 00451 AUGMENTIN 250 mg/5 mL 250 mg/5 mL NDC 43598-004-51 Directions for mixing: Tap bottle until all powder flows freely. Add approximately 2/3 of total water for reconstitution (total=65) shake vigorously to wet powder. Add remaining water, again shake vigorously. Dosage: See accompanying prescribing information. Keep tightly closed. Shake well before using. Must be refrigerated. Diecard after 10 days. Your answer AUGMENTIN® AMOXICILLIN/CLAVULANATE POTASSIUM FOR ORAL SUSPENSION When reconstituted, each 5 mL contains: AMOXICILLIN, 250 MG, as the trihydrate CLAVULANIC ACID, 62.5 MG, as clavulanate potassium 75 mL (when reconstituted) DR.REDDY'S Rx only Use only if inner seal is intact. Not contents:…
Demonstrate the procedure for arriving at the answer. A. It is necessary to carry out baths with Amitraz 12.5% in a batch of 25 patients. The product label says to dilute 1.5 ml of the product in 1 liter of water, for this there is a 50-liter water tank to prepare the product. Each patient corresponds to 1.2 L of the product diluted in water for an effective treatment. Calculate: a) total ml of amitraz needed to make the dilution in the water tank. b) ml of the product for each patient. c) Total ml of diluted Amitraz for the batch of patients. Answer: R: a) 75 ml. b) 1200 ml. c) 30.000 ml (Ctrl)-
6. You were asked by your laboratory instructor to prepare the following: 12 plates (use maximum volume) and 6 big slants of Malt Yeast Extract Glucose Agar. Calculate for the total volume of the media you need to prepare, and the amount (in grams) needed to be weighed for each component. Refer to the table below. Note: Show all computations. Use 3 significant figures for your final answer. Amount (per 200 ml) 0.6 g 0.6 g 4.0 g 3.4 g Malt Yeast Extract Glucose Agar Malt extract Yeast extract Glucose Peptone Agar 1.7 % Table 1. Glassware used in media preparation and their approximate volume capacity. Glassware Approximate volume* Petri dish 15-20 ml Big test tubes (18 mm diameter) as stabs/broth 10 ml as slants 6-8 ml Small tubes (13 mm diameter) as stabs/broth 5 ml as slants 3 ml It is prescribed not to exceed 60% of the total capacity of the container. Erlenmeyer flasks, media bottles, etc. *Volumes may vary depending on the laboratory practices.

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