Please answer questions 1 and 2 directly. 1. Discuss what is isoelectric precipitation/point?2. Differentiate whey from casein (refer from the posted picture) ISOLATION OF CASEINIsoelectric precipitation100ml milkIf full whole milk,proceed tocentrifugation. If not,proceed to pH meter forpH reading20g powder in 50ml waterHeatCheck for pH (6.6)CENTRIFUGATIONAdd 02N HCIFILTER & wash with water,diethyl ether & EtOHIEP pH 4.6Stand and settleResidue (dry& weigh)Filtrate(discard)eDiscard theupper layerSupernatantliquid(discard)Lower layer(protein)

The given diagram shows isolation by caesin by isoelectric precipitation

Answered: Please answer questions 1 and 2…

Please answer questions 1 and 2 directly. 1. Discuss what is isoelectric precipitation/point? 2. Differentiate whey from casein (refer from the posted picture)

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Please answer questions 1 and 2 directly. 1. Discuss what is isoelectric precipitation/point? 2. Differentiate whey from casein (refer from the posted picture)

ISOLATION OF CASEIN
Isoelectric precipitation
100ml milk
If full whole milk,
proceed to
centrifugation. If not,
proceed to pH meter for
pH reading
20g powder in 50ml water
Heat
Check for pH (6.6)
CENTRIFUGATION
Add 02N HCI
FILTER & wash with water,
diethyl ether & EtOH
IEP pH 4.6
Stand and settle
Residue (dry
& weigh)
Filtrate
(discard)e
Discard the
upper layer
Supernatant
liquid
(discard)
Lower layer
(protein)

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