5. Given the following TLC and melting point data for three unknown samples sample melting point (°C) TLC Unknown 1 99-100 Unknown 2 110-112 Unknown 3 110-112 What is the best conclusion to draw about whether the samples are the same or different? Explain your reasoning. Three to 5 simple sentences is enough to do this. NOTE ON QUESTION 5 You should decide on possible conclusions based on the data given and your knowledge of the techniques. Some conclusions will of course be better than others, and so you must look at all possibilities and pick the most reasonable possibility and discuss that one in your answer. Lane 1 (left lane) Lane 2 (middle lane) Lane 3 (right lane) TLC Analysis for Question 5
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- The dilution factor for Sample 1 if 20 µL of extract is added to 980 µL distilled water is 50. What would the dilution factor for Sample 1 be if 500 µL of the diluted sample from last sentence were diluted with 500 µL distilled water?A flat (not spherical) tissue engineered skin (thickness 3.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.2 E-9 m?/s, and according to experiments that you've performed, you want to make sure that the concentration of oxygen in the device doesn't fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5 E-17 mol/[cell•s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?2.1The number of bacteria in the samples need to be quantified. The laboratory uses a specificprotocol where the dilution factor on the spread plates must be:10-6; 10-7; 10-8Describe how you would prepare the required dilution series using the least amount of dilutionwater. 2.2 Calculate the amount of bacteria in the sample from the following results (show the steps in the calculation): Dilution factor Count10-6 27110-7 3510-8 21
- A biochemist carefully measures the molarity of salt in 56. mL of photobacterium cell growth medium to be 82. mM. Unfortunately, a careless graduate student forgets to cover the container of growth medium and a substantial amount of the solvent evaporates. The volume of the cell growth medium falls to 3.1 mL. Calculate the new molarity of salt in the photobacterium cell growth medium. Be sure your answer has the correct number of significant digits. | mM10 If3 ml of culture is diluted by adding 9 ml of water and the absorbancy reading of the diluted culture is 0.082, what is the actual absorbancy?7. A total of 7.21 g of dry mixture with a protein content of 89.2% (w/w) determined by Kjeldahl analysis was dissolved in 88.54 g of sterile water and centrifuged to purify the protein in the mixture. After centrifugation, a total of 5.95 g of precipitated pellet was formed, and the protein content in pellet was determined as 72.2% by spectrophotometric Lowry analysis. Assume that pellet is not a hygroscopic material and does not absorb any water. Determine the following: a. the protein concentration in the supernatant layer after centrifugation in % w/w b. the nature of amino acids in the protein structure. What's the possible explanation?
- A flat (not spherical) tissue engineered skin (thickness 2.5 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.4 E-9 m2/s, and according to experiments that you’ve performed, you want to make sure that the concentration of oxygen in the device doesn’t fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5.9 E-17 mol/[cell◦s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.636, taking into account the dilution factor as per question 6 (100ul) and the total volume of extract as per question 4 (140ml). Give your answer as a number with no decimal places.Figure 3: ● ● ● ● ● ● KDa ● 97.4 66.2 45.0 ● 31.0- 21.5 14.4 S-1 p-1 S-2 2-0 This figure was generated by centrifuging a pura sample of protein, removing the supernatant, and resuspending the pellet in the same volume as the supernatant to allow direct comparison. The supernatant and pellet samples were then prepared for SDS-PAGE identically and run via normal SDS-PAGE procedures. In the figure, "s" means supernatant and "p" means pellet. The text or number after the dash represents a different condition. For example, s-1 and p-1 are the supernatant and pellet samples under condition 1. It is not shown, but under wild-type conditions, essentially all of the protein is found in the supernatant. S-3 ● What does the intensity of each band represent? ● Would you find soluble protein in the supernatant or pellet? Why? Would you find aggregated protein in the supernatant or pellet? Why? For each condition (there are 5 different conditions), is there a higher percentage of the total protein…
- The number of cells in a culture is estimated based on turbidity. If the following standards are used for comparison: Number of cells/mL Optical Density (measure of turbidity) 0.025 0.050 000'000'৮ 000 000'000' కరరక How many cells are present in a culture that has an optical density of 0.075? O A. Less than 1,000,000 O B. Between 2,000,000 and 4,000,000 O C. 16,000,000 O D. Between 4,000,000 and 8,000,000 Reset SelectionThe decomposition reaction of atmospheric peroxyacetylnitrate (PAN) to products was studied, and the following rates were obtained. Temperature, °C Rate constant, s1 0.00 5.60 x 10-6 10.0 3.20 x 10-5 20.0 1.60 x 10 4 30.0 7.60 x 10-4 43. Fit this data into the Arrhenius equation. Give the values of the following: Slope = ( Select ) y-intercept = I Select] Pearson coefficient (R) = [ Select ] activation energy of this reaction in kJ/mol = [Select ] collision frequency factor A = I Select]Your pharmacy has on hand Tetracycline 250 mg/5 mL liquid.You receive a prescription for Tetracycline syrup 500 mg qid for 7 days. How many total milliliters would you dispense? Your pharmacy has on hand Prednisone 5 mg tablets.You receive a prescription for Prednisone 5 mg ii tabs stat and qid x 1 day, then ii tabs tid x 2 days, then ii tabs bid x 2 days, then i tab tid x 3 days, then i tab bid x 3days, then i tab qd x 4 days How many tablets would you dispense for this prescription? How many days will the patient take this medication for? Your pharmacy has on hand Dilantin 100mg capsules.You receive a prescription for Dilantin 100 mg 1 cap po in the AM & PM, 200mg (2 caps) po at noon, and 300mg (3 caps) po at hs. Dispense a 30 day supply. (using the 100mg capsules you have in stock) How many capsules will you dispense? Your pharmacy has on hand Dakins Irrigation Solution 1 liter.You receive a prescription for Dakins Irrigation Solution Irrigate chest tube with 100 mL…