Answer no 2 only in the problem 9788801. After blood collection, the red cells are separated from the serum to be used for the preparation of the stocksolution. How is it done?2.3.DF: 4For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must beadded to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s.What is the appropriate diluent used for the preparation of the red cell suspension?How much of the diluent must be added to Tube 2 if 200uL RCS is added? Show pertinent solution/s.4.5.What are the initial concentrations used for tubes 3, 5 and 6? Show pertinent solution/s.6. What are the dilutions of the last positive tube and first negative tube respectively? Show computation as well.7. If the red cell suspension is the stock solution, what is being quantitated in this test?8.What diluent/reactant should be used to detect your answer in number 7?9. PIPETTING TECHNIQUES: Enumerate 3 errors that would lead to inaccurate dispensing of desired volume10. Give at least 1 advantage of serologic pipette to automatic pipette (A) and vice versa (B)

Here we are trying to prepare the stock solution from the raw red cell (RBC) solution. The parameter…

Answered: For the serial dilution, your stock…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Remaining V Ha Question 17 Triose phosphate isomerase catalyzes the conversion of Glyceraldehyde-3- phosphate to Dihydroxyacetone phosphate. Glyceraldehyde-3-P Dihydroxyacetone phosphate The Km of this enzyme for its substrate Glyceraldehyde-3-phosphate is 1.8 x 10 M. When [Glyceraldehyde-3-phosphate] = 30 uM the rate of the reaction (v) was 82.5 umol/mL/sec and the Vmax was 132 umol/mL/sec. Assuming 3 nanomoles/mL of enzyme was used ([Eotal = 3 nanomol/mL) the kcat for this enzyme is: Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 44000/sec b 1100/sec 88/min d. 120/hour e 11/min Im f 450000/sec g 52800/sec 175/sec
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Question #2: Consider the following data for Cell G run in 1 L chemostat, with a feed containing 30 g/L substrate: Feedrate (ml/hr) (c) Calculate 27.5 24.2 22.1 18.8 Steady State Cell Mass Concentration (g/L) 12.0 14.7 15.8 16.7 Steady State Substrate Concentration (g/L) 10.0 5.56 (d) Using the calculated kinetic parameters, 3.70 (a) Determine the kinetic parameters for growth (b) At each dilution rate, calculate Yx/s. Is it constant with dilution rate and substrate concentration? 2.23 (i) the critical dilution rate, D. (ii) the dilution rate corresponding to maximum productivity, Dm (iii) the maximum cell productivity (cells/L/hr) (i) Generate a plot of X and S vs D for a feed concentration of 25 g/L. (ii) What is the critical dilution rate for this condition? (iii) What is the dilution rate corresponding to maximum productivity at this condition? (iv) What is the maximum cell productivity at this condition? (v) For a reactor operating at this maximum productivity, what would be the…
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2
Rotate the ball horizontally on an 80 cm long non-stretchable cord with angular velocity of 3 s^(-1). After ten seconds of clockwise rotation, the cord breaks. At what speed and in what direction does the ball fly, if it was faced north at time t = Os? Where and after how much time does the ball land on the ground that is 1 m below the plaine in which we rotate the string? {Solution: v= (0.37 m/s,2.37 m/s); d=1.07 m, t=0.447 s.) }

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For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s.