A: State the Lineweaver Burke equation. B: Calculate the kinetic parameters Vmax and KM from the figure. C: Estimate the turnover number of the enzyme.
A: State the Lineweaver Burke equation. B: Calculate the kinetic parameters Vmax and KM from the figure. C: Estimate the turnover number of the enzyme.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Another exam question in biological chemistery from last year. I have an exam in biological chemistery this month, so I try to be prepared. I'm trying to figure out how to calculate WITHOUT using computer (its not allowed during exam).
I will appreciate details when explaining this exercise, so I can understand it. Thank you
![The kinetics of an enzyme have been investigated experimentally. Some of the results are
given in the figure below in the form of a Lineweaver-Burk plot (sometimes called a double
reciprocal plot). In this experiment, the enzyme concentration was 11.5 nM (i.e. 1.15 x 10-8
M).
1/V (s/mM)
2500
2000
1500
1000
500
0
ODDO
0
100
200
1/[S] mM-1
300
400](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F276c085d-4976-4cc2-ba9f-ed8f400f3309%2F93026e2f-0f76-4853-b626-1f986dbc425a%2F6bxotvm_processed.png&w=3840&q=75)
Transcribed Image Text:The kinetics of an enzyme have been investigated experimentally. Some of the results are
given in the figure below in the form of a Lineweaver-Burk plot (sometimes called a double
reciprocal plot). In this experiment, the enzyme concentration was 11.5 nM (i.e. 1.15 x 10-8
M).
1/V (s/mM)
2500
2000
1500
1000
500
0
ODDO
0
100
200
1/[S] mM-1
300
400
![A: State the Lineweaver Burke equation.
B: Calculate the kinetic parameters Vmax and KM from the figure.
C: Estimate the turnover number of the enzyme.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F276c085d-4976-4cc2-ba9f-ed8f400f3309%2F93026e2f-0f76-4853-b626-1f986dbc425a%2Fjqhjz3h_processed.png&w=3840&q=75)
Transcribed Image Text:A: State the Lineweaver Burke equation.
B: Calculate the kinetic parameters Vmax and KM from the figure.
C: Estimate the turnover number of the enzyme.
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Follow-up Question
Thank you for helping me. I have to questions. In solution B, if we choose two other points on the graph for calculating the slope, wouldnt the values for Km/Vmax be different than 6.1? Why did you choose exactly those points on the graph?
Another thing, I understand the method you used to get to Km/Vmax= slope, but how did you then get the value for Vmax to be 10 mM/s??
Solution
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