The Effect of Various Factors on the Rate of an Enzyme Reaction. These experiments will use the enzyme catalase, which is found in potatoes. Catalase acts on the substrate, H2O2 (hydrogen peroxide), to produce H2O and O2. The O2 is released as bubbles. Therefore, the more bubbles you see, the greater the reaction.Base your responses on the following data table:The effect of pH on the rate of enzyme reactionsTubepHPrediction- no bubbles+ some bubbling++ strong bubbling+++ very strong bubblingObservation- no bubbles+ some bubbling++ strong bubbling+++ very strong bubbling13--25--37+++49-+++511-+ Relate this to protein structure and denaturization. Again, thinking of the effect of pH on enzyme activity, why does the pancreas secrete HCO3-?What would you predict to happen to digestion and absorption in the small intestine if the pancreas stopped producing bicarbonate?

The effect of pH on enzyme (catalase) activity was studied and following results were obtained.…

Answered: The Effect of Various Factors on the…

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The Effect of Various Factors on the Rate of an Enzyme Reaction. These experiments will use the enzyme catalase, which is found in potatoes. Catalase acts on the substrate, H₂O₂ (hydrogen peroxide), to produce H₂O and O₂. The O₂ is released as bubbles. Therefore, the more bubbles you see, the greater the reaction.

Base your responses on the following data table:

The effect of pH on the rate of enzyme reactions

Tube	pH	Prediction - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling	Observation - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling
1	3	-	-
2	5	-	-
3	7	+	++
4	9	-	+++
5	11	-	+

Relate this to protein structure and denaturization. Again, thinking of the effect of pH on enzyme activity, why does the pancreas secrete HCO₃^-?
What would you predict to happen to digestion and absorption in the small intestine if the pancreas stopped producing bicarbonate?

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