I have an exam in biological chemistery this month, so I try to be prepared. This is an exam question from last year, that I'm trying to figure out how to calculate WITHOUT using computer (its not allowed during exam). I will appreciate details when explaining this exercise, so I can understand it. Thank you The enzyme Lysozyme is present in human nasal mucus and catalyzes the breakdownof a component of the cell wall of certain bacteria, causing them to burst. Hence,Lysozyme helps protect against bacterial infections. One experimental investigation ofLysozyme gave the result in Table 1. Here, [S]o is the initial substrate concentrationand V is the initial rate of the breakdown reaction.[S]o(µM)1.02.04.01020Table 1V(µM/sec)0.140.220.310.420.48Use a graphical method to determine the Michaelis constant, Kè, and the maximal rate,V max.What initial reaction rate would be expected for [S]0=200μM?In these experiments the concentration of lysozyme was 0.0025 µM. Calculate themaximal turnover, kcat, for lysozyme under the investigated conditions.Lysozyme belongs to Enzyme Class number 3 (EC 3), the so-called hydrolases. Whatcharacterizes a hydrolase?

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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I have an exam in biological chemistery this month, so I try to be prepared. This is an exam question from last year, that I'm trying to figure out how to calculate WITHOUT using computer (its not allowed during exam).

I will appreciate details when explaining this exercise, so I can understand it. Thank you

The enzyme Lysozyme is present in human nasal mucus and catalyzes the breakdown
of a component of the cell wall of certain bacteria, causing them to burst. Hence,
Lysozyme helps protect against bacterial infections. One experimental investigation of
Lysozyme gave the result in Table 1. Here, [S]o is the initial substrate concentration
and V is the initial rate of the breakdown reaction.
[S]o
(µM)
1.0
2.0
4.0
10
20
Table 1
V
(µM/sec)
0.14
0.22
0.31
0.42
0.48
Use a graphical method to determine the Michaelis constant, Kè, and the maximal rate,
V max.
What initial reaction rate would be expected for [S]0=200μM?
In these experiments the concentration of lysozyme was 0.0025 µM. Calculate the
maximal turnover, kcat, for lysozyme under the investigated conditions.
Lysozyme belongs to Enzyme Class number 3 (EC 3), the so-called hydrolases. What
characterizes a hydrolase?

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Thank you for taking the time to explaining this exercise. however, as I mentioned earlier I'm not allowed to use computer during exam, therefor the answer need to be determined without using any programmes. So, how can I do it? because your method can only be done if you are using excel.

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