I need help calculating the “Activity ([PNPP]) for each Supernatent. I need to know how to solve this so I can fill out my purification table worksheet. Please help. This class is Biochemistry 1 Lab. **Purification Table Part 2****Overview:**For steps 3 through 8, you will use Acid Phosphatase assays to determine the amounts of the enzyme in your supernatants.---**Preparation and Dilutions:**Prepare the following dilutions of each supernatant to determine the phosphatase activity:| Supernatant | Dilution | Volume Sup (µL) | H2O (µL) | Total Volume (µL) ||-------------|----------|------------------|----------|------------------|| V | 1/100 | 1.5 | 498.5 | 500 || VI | 1/200 | 7.5 | 142.5 | 150 || IV | 1/200 | 7.5 | 142.5 | 150 || II | 1/100 | 7.5 | 142.5 | 150 || I | 1/25 | 9 | 441 | 450 || VI (blank) | 1/750 | 9 | 141 | 450 |*Note*: You need at least 0.2 ml final volume for each fraction.---**Assay Reaction Preparation:**1. Prepare the assay reaction for each fraction, plus a control containing no protein.2. Create a mix containing the chemicals below. Due to pipeting errors, prepare a mix for eight tubes but use seven tubes.3. Aliquot 0.3 ml of the mix into each of the seven tubes (add closest Sups).*Master Mix Total Volume: 3000 µL*| Reagent | Volume/tube (mL) | Volume in mix (mL) ||------------------|------------------|--------------------|| 1.0 M Sodium acetate | 0.05 | 0.4 || 1.0 M MgCl2 | 0.05 | 0.4 || H2O | 0.20 | 1.6 |---**Assay Execution:**5. To each of the assay reactions, add 150 µL of either the appropriate diluted fraction (from step 3, one per tube) or H

Here we are undertaking the acid phosphatase assay. Acid phosphatase enzyme catalyzes the hydrolysis…

Answered: calculating the “Activity ([PNPP]) for…

Science

Biochemistry

calculating the “Activity ([PNPP]) for each Supernatent.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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