Question Completion Status: 2,0 1,6- 1,2 0,8 0,4 2000- CH₂ 31 CH-COO 260 300 340 400 nm If you wanted to follow the activity of the enzyme isocitrate dehydrogenase (shown below) what would be the best way to do this this? 21 HO-C-H 31 NADH NAD COO Isocitrate Wavelength AG = -8.4kJ/mol AG'=-17kJ/mol NAD NADH HP CO₂ CEO COO a-ketoglutarate a. Follow the increase in NADH concentration by following the increase in absorbance at 340nm. Ob. Follow the increase in NAD concentration by following the increase in absorbance at 260nm. Oc. Follow the decrease in NADH concentration by following the decrease in absorbance at 260nm. Od. Follow the increase in NAD* concentration by following the increase in absorbance at 340nm.
Question Completion Status: 2,0 1,6- 1,2 0,8 0,4 2000- CH₂ 31 CH-COO 260 300 340 400 nm If you wanted to follow the activity of the enzyme isocitrate dehydrogenase (shown below) what would be the best way to do this this? 21 HO-C-H 31 NADH NAD COO Isocitrate Wavelength AG = -8.4kJ/mol AG'=-17kJ/mol NAD NADH HP CO₂ CEO COO a-ketoglutarate a. Follow the increase in NADH concentration by following the increase in absorbance at 340nm. Ob. Follow the increase in NAD concentration by following the increase in absorbance at 260nm. Oc. Follow the decrease in NADH concentration by following the decrease in absorbance at 260nm. Od. Follow the increase in NAD* concentration by following the increase in absorbance at 340nm.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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a. Follow the increase in NADH concentration by following the increase in absorbance at 340mm.
O b. Follow the increase in NADt concentration by following the increase in absorbance at 260nm.
c. Follow the decrease in NADH concentration by following the decrease in absorbance at 260nm.
d. Follow the increase in NAD* concentration by following the increase in absorbance at 340nm.
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