Answered: From the chart below, comment on the… | bartleby

Curren'S Math For Meds: Dosages & Sol

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter21: Heparin Infusion Calculations

Section: Chapter Questions

Problem 1.4P

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Question

From the chart below, comment on the effect of substrate concentration on the rate of reaction.

3. THE EFFECT OF SUBSTRATE CONCENTRATION
30 seconds s
IT
Tube
#
1
12
3
4
5
6
7
18
Substrate
10
conc.
40µg/ml
40µg/ml
80µg/ml
80µg/ml
120µg/ml
120µg/ml
160µg/ml
160µg/ml
Abs.
200µg/ml
200µg/ml
N/A
0.340
N/A
0.363
N/A
0-391
N/A
0.537
Comment on the effect of substrate concentration.
0.381
N/A

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Table 1 - Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract Absorbance 0 mins after 30mins (2nd reading) (mL) 1st reading 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 0.00 0.060 0.033 0-05-2 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1? Time for reading: 3:21 -0.11 Absorbance: Time for reading: 3.36 Q5) Tube la serves as a control, but why is this control needed? Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: catechol Q2) What is the substrate? Answer: THO Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: . 166 ness Catechol Benzoquinone Subtract 1st from 2nd reading -0.01 0-137 0.11.19 0.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different…
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