Linearize the equations: Umaz C kis + C v = UmaxC² V = k? + C²
Chapter8: Have A Heart
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Problem 4FITB
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Linearize the equations
Transcribed Image Text:QUESTION III:
Laboratory tests have been conducted for a specific enzyme in which the enzyme concentration
(C) has been varied between 0 – 10 and the initial reaction velocity (v) is measured. The results
are similar to Figure 1. The raw data for the tests are also supplied in a separate Excel file. You
should use the Excel sheet corresponding with your "B" number.
0.45
0.4
0.35
000
0.3
0.25
0.2
0.15
0.1
0.05
2.
7.
10
Figure 1. Experimental C vs v data.
The Michealis-Menten equation (Eq. 1) is commonly used to describe enzyme reaction kinetics:
Vm, C
V max
V =
(1)
k, +C
Where Vmax is the maximum initial reaction velocity, ks is the half-saturation constant and C is
the substrate concentration.
Transcribed Image Text:Linearize the equations:
Umax C
kis + C
v =
Umax C2
V =
k + C2
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