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primary and metastatic uveal Mel202 and OMM1.3 cells.

primary and metastatic uveal Mel202 and OMM1.3 cells.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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3. Imagine you performed the BCA assay on your experimental samples (extracted protein lysates from primary and metastatic uveal melanoma cell lines). Your experimental samples were lysates from Mel202 and OMM1.3 cells. You obtain the following absorbance samples for each lysate. Use the line of best fit that you obtained in question 2 above to calculate the final concentration of protein in each of these samples. (Assume that any dilutions from the procedures are already factored into the calculations. Just use the equation of the line to solve for the final protein concentration of each lysate directly). Show your work in the space below each answer. Absorbance at 562 nm for Mel202 lysate = 1.324 Protein Concentration for Mel202 lysate = Work for the calculation above: Absorbance at 562 nm for OMM1.3 lysate = 0.376 Protein Concentration for OMM1.3 lysate = Work for the calculation above: ug/ml ug/ml 4. In the subsequent lab you would have loaded your protein lysates into an SDS-PAGE gel. Imagine you have space in each well of the gel to load 25 ul volume of protein lysate. What would be the total concentration of each lysate that is loaded into their well if you load just 25 ul of each? Well #1: Concentration of protein in Mel202 lysate that is equivalent to 25 ul volume = Work for the calculation above: Well #2: Concentration of protein in OMM1.3 lysate that is equivalent to 25 ul volume = Work for the calculation above: ug ug
Transcribed Image Text:3. Imagine you performed the BCA assay on your experimental samples (extracted protein lysates from primary and metastatic uveal melanoma cell lines). Your experimental samples were lysates from Mel202 and OMM1.3 cells. You obtain the following absorbance samples for each lysate. Use the line of best fit that you obtained in question 2 above to calculate the final concentration of protein in each of these samples. (Assume that any dilutions from the procedures are already factored into the calculations. Just use the equation of the line to solve for the final protein concentration of each lysate directly). Show your work in the space below each answer. Absorbance at 562 nm for Mel202 lysate = 1.324 Protein Concentration for Mel202 lysate = Work for the calculation above: Absorbance at 562 nm for OMM1.3 lysate = 0.376 Protein Concentration for OMM1.3 lysate = Work for the calculation above: ug/ml ug/ml 4. In the subsequent lab you would have loaded your protein lysates into an SDS-PAGE gel. Imagine you have space in each well of the gel to load 25 ul volume of protein lysate. What would be the total concentration of each lysate that is loaded into their well if you load just 25 ul of each? Well #1: Concentration of protein in Mel202 lysate that is equivalent to 25 ul volume = Work for the calculation above: Well #2: Concentration of protein in OMM1.3 lysate that is equivalent to 25 ul volume = Work for the calculation above: ug ug
2. The following data were generated after a set of bovine serum albumin standards were measured via the BCA assay. Table 1. Absorbance measurements at 562 nm for BSA standards measured in triplicate in a BCA Assay. Standards A B C D E F G H I Conc (ug/ml) 2000 1500 1000 750 500 250 125 25 0 Replicate 1 Abs @ 562 nm 2.381 2.039 1.505 1.143 0.819 0.454 0.245 0.056 0 Replicate 2 Abs @ 562 nm 2.456 2.052 1.489 1.156 0.823 0.46 0.272 0.049 0 Replicate 3 Abs @ 562 nm 2.43 2.05 1.47 1.17 0.81 0.48 0.255 0.053 0.00 Use Excel or your graphing program of choice to create a standard curve using the data from table 1. Consider the best way to graph this data. Make sure the type of graph that you select will allow you to add a line of best fit. Display the equation for the line of best fit directly on the graph. Hint... you'll want to think about how to treat the triplicate values when making the graph. Also consider the type of graph that should be selected to allow for addition of the line of best fit. Copy and paste your graph below (or paste a screenshot image of your graph). Add a detailed scientific caption to your figure.
Transcribed Image Text:2. The following data were generated after a set of bovine serum albumin standards were measured via the BCA assay. Table 1. Absorbance measurements at 562 nm for BSA standards measured in triplicate in a BCA Assay. Standards A B C D E F G H I Conc (ug/ml) 2000 1500 1000 750 500 250 125 25 0 Replicate 1 Abs @ 562 nm 2.381 2.039 1.505 1.143 0.819 0.454 0.245 0.056 0 Replicate 2 Abs @ 562 nm 2.456 2.052 1.489 1.156 0.823 0.46 0.272 0.049 0 Replicate 3 Abs @ 562 nm 2.43 2.05 1.47 1.17 0.81 0.48 0.255 0.053 0.00 Use Excel or your graphing program of choice to create a standard curve using the data from table 1. Consider the best way to graph this data. Make sure the type of graph that you select will allow you to add a line of best fit. Display the equation for the line of best fit directly on the graph. Hint... you'll want to think about how to treat the triplicate values when making the graph. Also consider the type of graph that should be selected to allow for addition of the line of best fit. Copy and paste your graph below (or paste a screenshot image of your graph). Add a detailed scientific caption to your figure.
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