Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 2A
Why did the use of DNA polymerase from the bacterium Thermus aquaticus allow researchers to add the necessary reagents to tubes in a preprogrammed heating block?
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Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Why is the DNA polymerase used in PCR derived from an extreme thermophile bacteria species rather than a mesophile bacteria species? (be sure to describe the mesophile DNA polymerase as well as the extreme thermophile DNA polymerase.)arrow_forwardWhat is PCR? Why does Taq polymerase work better than a typical DNA Polymerase isolated from E. coli for PCR? The optimal growth temperature for E Coli is 37 °C.arrow_forwardWhy is MgCl2 added to the PCR reaction?arrow_forward
- You culture bacteria from the soil at a toxic waste dump on an agar plate and pick a single bacterium from which you prepare DNA. The isolated organism is a new, previously uncharacterized species. You perform PCR amplification of this DNA using primers to amplify a short segment of ribosomal DNA. When you use BLAST to find similar sequences, you find the following results. Organism E. coli Streptococcus Burkhoderia Microbacterium oxydans DNA sequence identity. % sequence E-value coverage e-10 E. coli 84% 88% 98% 100% 40% 40% 40% 80% e-12 e-15 e-30 Based on the data in the table, which species is most closely related to your toxic waste dump sample? Microbacterium oxydans Streptococcus Burkhoderiaarrow_forwardWhy do you need to put the reagents in the reaction tubes into the tubes in a specific order for DNA fingerprint analysis? Explain what the order , and why it was necessary.arrow_forwardWhat is the purpose of the blank test in the experiments?arrow_forward
- What is the most important thing to remember when working with PCR reagents in terms of temperature control?arrow_forwardPlease answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?arrow_forwardwhy different thermal conditions are necessary for successful PCR?arrow_forward
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