Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 2A
Why did the use of DNA polymerase from the bacterium Thermus aquaticus allow researchers to add the necessary reagents to tubes in a preprogrammed heating block?
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tudent forgot to add GelRed in to the gel mixture when he prepared the Agarose Gel. What will be the problem caused by his mistake?
A) He will not be able to securely deposit his samples in the wells, the samples will disperse into the buffer
B) The student will have a hard time visualizing how far his samples have traveled in the gel to disconnect the power supply in time
C) The student will not be able to visualize the DNA under the UV light
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Which of the following is true of the gel electrophoresis shown? Note: Only one can be true... I'm between c or d. Previous answer confused me more.
A) the power source has not been turned on yet.
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D) well #3 had more different sized molecules of DNA than well #1 or #2
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Why is the DNA polymerase used in PCR derived from an extreme thermophile bacteria species rather than a mesophile bacteria species? (be sure to describe the mesophile DNA polymerase as well as the extreme thermophile DNA polymerase.)arrow_forwardWhat is PCR? Why does Taq polymerase work better than a typical DNA Polymerase isolated from E. coli for PCR? The optimal growth temperature for E Coli is 37 °C.arrow_forwardWhy is MgCl2 added to the PCR reaction?arrow_forward
- Why is polyacrylamide gel is used in electrophoresis?arrow_forwardDescribe how would you determine the following using a real-time PCR instrument. A) The quantity and quality of a DNA sample. B) Whether an individual had cystic fibrosis from a genomic DNA sample. C) If several bacterial pathogens were present in DNA extracted from a food sample.arrow_forwardWhat would happen if the enrichment method in the isolation of bacteriophage was omitted?the chloroform was not added to the enrichment?the 0.1 ml lysate - E.coli mix was plated directly on top of the bottom agar?arrow_forward
- You followed all the steps correctly with a kit you bought last week. However, NO purified DNA came out of the miniprep (More than one answer possible). a) The plasmid was lost during culture growth b) Your tears from doing the same experiment again and again contaminated the reagents, causing them not to work c) Depurination of the DNA led to loss of genetic material d) Incomplete lysis of the cells after adding lysis bufferarrow_forwardWhy were radioactive sulfur and phosphorous used to label bacteriophage in Hershey and Chase's experiments?arrow_forwardThe following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.arrow_forward
- Why can the Chelex solution extract DNA in just one step?arrow_forwardName the substance used as a medium in gel electrophoresis.arrow_forwardUse the plasmid to answer the following questions a) Digest the plasmid with EcoRI only and then both EcoRI and BamHi together. Fill in the table to show the fragment sizes produced.arrow_forward
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