Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 5MCQ
If you put a gene in a virus, the next step in genetic modification would be
- a. insertion of a plasmid.
- b. transformation.
- c. transduction.
- d. PCR.
- e. Southern blotting.
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Students have asked these similar questions
Which of the following is true?
a.
Viruses have been used to introduce certain genes into humans.
b.
PCR can be used in tests for inherited diseases
c.
All the above
d.
DNA chips can carry sequences from over 1000 gene sequences on one chip.
Which of the following characteristics is not true of a plasmid?a. It is a circular piece of DNA.b. It is required for normal cell function.c. It is found in bacteria.d. It can be transferred from cell to cell.
Which of the following is defined as transformation?
A. Transfer of genetic information via a plasmid
B. Virus infects a host and deposits genetic material
C. Free floating DNA is uptaken
D. Transfer of genetic information from one bacterium to another
5.
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- after a successful infection by a viral particle, it integrates its genome into the host DNA. which term best describe the change of host genome? a. none b.silent mutation c.missense mutation d.frame shift mutationarrow_forwardBacteria are able to successfully transcribe and translate humangenes becausea. both bacteria and humans contain plasmid vectors.b. bacteria can replicate their DNA, but humans cannot.c. human and bacterial ribosomes are vastly different.d. the genetic code is nearly universal.arrow_forwardhas been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a Transgenic genome b. Recombinant DNA sequence C. Knockdown gene d. Synthetic genome e. Recombinant plasmid xarrow_forward
- Plasmids are circular extrachromosomal DNA in bacteria that play a role in: d. degradation of viruses a. growth of bacteria b. pathogenicity and antibiotic resistance c. production of antibodiesarrow_forwardWhich of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.arrow_forwardIn gene therapy, healthy genes are placed into animal cells that have defective genes by usinga. a DNA particle gun.b. micropipettes (needles).c. virus vectors.d. Cells are not modified genetically. Instead, healthyarrow_forward
- The polymerase chain reaction (PCR) is important because it allows us to Group of answer choices a. insert eukaryotic genes into prokaryotic plasmids b. incorporate genes into viruses c. make DNA from RNA transcripts d. make many copies of a target segment of DNAarrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forwardWhich of the following may be used as a vector in a gene-cloningexperiment?a. mRNA c. Virusb. Plasmid d. Both b and carrow_forward
- An experimental drug therapy to treat patients with antibiotic-resistant bacteria involves introduction of a highly specific bacteriophage to the infected patient's bloodstream. Which of the following bacteriophage types would be the LEAST useful for this therapy? a. a lytic bacteriophage b. An enveloped virus c. An RNA virus d. a lysogenic bacteriophagearrow_forwardA small nonessential circular DNA molecule in prokaryotes is called a: a. plasmid b. telomere c. bacteriophage d. prophagearrow_forwardWhich of the following describes sequencing by synthesis? A. Large sections of DNA are inserted into a plasmid. B. DNA sequence is recorded as the complementary strand is built. C. Many copies of the DNA are made, each terminating at a specific nucleotide. D. A DNA strand is forced through a pore and its sequence synthesized as it passes through.arrow_forward
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