Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 2R
Differentiate the following terms. Which one is “hit and miss”— that is, does not add a specific gene to a cell?
- a. protoplast fusion
- b. gene gun
- c. microinjection
- d. electroporatio
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Choose the one answer that fits best. Which of the following statements regarding bacteria is NOT correct?
a.
During conjugation, two bacteria exchange genetic information directly via pili
b.
Bacterial populations have a higher mutation rate because their DNA Polymerases do not have proofreading capability
c.
Bacterial growth by binary fission is exponential and each division usually takes only a couple of hour or less
d.
Plasmids carry just a few genes and are replicated independently from the bacterial chromosome
e.
Cocci that are arranged in clusters/bunches are called staphylococci
3) Examine the graph showing the relative percentage normal and cancer cells spend in various stages of the cell cycle. Based on the information in the graphs, infer how cancer cells differ from typical, noncancerous cells. Select ALL that apply.
A) Cancer cells do not replicate their DNA.
B) Cancer cells replicate their DNA too quickly.
C) Cancer cells do not go through interphase during their cell cycle.
D) Cancer cells spend more time dividing compared to typical cells.
E) Cancer cells do not always grow to the same size as typical cells.
more than 1 answer. not graded
A. Directions: Label the diagram involved in the process of genetic engineering below. Use
these choices:
recombinant DNA
plasmid-containing bacterium
DNA fragment
bacterial cell (host)
cell division
DNA ligase
plasmid (vector)
clone
+
1.
00
7.
00
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Transformation of mammalian cells with viruses and obtaining mammalian cells harboring the virus is a method used to maintain the virus of interest. During this process what are the plaques observed? A. Clear areas in a layer of cultured cells degenerated and lysed due to virus infection. B. Stained areas in a cell culture indicating cells infected by a virus. C. Virus colonies on agar. D. Bacterial colonies on agararrow_forwardMatch description with Term: 1) determine the amount of protein in a sample 2) determine the location of a protein in a cell or sample 3) determine the amount of transcript in a sample 4) Determine the location of a transcript in a sample or cell a. In situ hybridization b. northern blotting c. western blotting d. immunocytochemistryarrow_forwardThe advantage of yeast cells over bacterial cells to express human proteins is that: a. yeast cells grow faster b. yeast cells are easier to manipulate genetically c. yeast cells are eukaryotic and modify proteins similarly to human cells d. yeast cells are easily lysed to purify the proteinsarrow_forward
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