Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 2R
Differentiate the following terms. Which one is “hit and miss”— that is, does not add a specific gene to a cell?
- a. protoplast fusion
- b. gene gun
- c. microinjection
- d. electroporatio
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5) Below is an image that shows both reproductive and therapeutic cloning. Use this image to answer compare and contrast therapeutic and reproductive cloning. Are they used for similar means…etc. Once you have done that answer the question below.
a) There are two types of therapeutic cloning. What are they and how are they different?
give An overview. on Gene transfer in bacteria:
Match description with Term:
1) determine the amount of protein in a sample
2) determine the location of a protein in a cell or sample
3) determine the amount of transcript in a sample
4) Determine the location of a transcript in a sample or cell
a. In situ hybridization
b. northern blotting
c. western blotting
d. immunocytochemistry
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Describe the process of somatic cell nuclear transfer and how it is used to create cloned cells. Explain what you consider to be the positive and negative aspects of cloning both scientifically and morally, giving at least three ‘for’ and three ‘against’ points – Discuss whether you think it is a good use of science and will benefit mankind or whether you believe it is unethical and should no longer be pursued?arrow_forward_________ is an example of reproductive cloning. a. Somatic cell nuclear transfer SCNT b. Multiple offspring from the same pregnancy c. Artificial embryo splitting d. a and carrow_forwardYou have found a gene of interest in an animal in the rain forest which produces a unique protein that will blocks reproduction of the pathogen that now plagues the world. You wish to mass produce this protein using bacteria. Answer the following questions regarding the scenario above. Describe how you would remove the gene of interest out of the isolated animal cell. What type of vector, (delivery system), would be best used to get the gene into the bacteria? How would you insert the gene of interest into the vector? How would get the vector into the bacteria? Why would the bacteria express this gene even if it normally does not have use for this protein?arrow_forward
- Make a flow chart of the basic process of gene therapy. Explain each part of the process.arrow_forwardYou are determined to produce an antiserum for the next pandemic that has hit the world. You have found a gene of interest in an animal in the rainforest which produces a unique protein that blocks the reproduction of the pathogen that now plagues the world. You wish to mass produce this protein using bacteria.arrow_forwardReview the description of Fred Griffith's experiments with Streptococcus pneumoniae. Using your knowledge of horizontal gene transfer, explain the process by which the harmless bacteria became dangerous after exposure to components of harmful bacterial cells.arrow_forward
- Briefly, explain specialized transduction and conjugation.arrow_forwardAnswer the following questions:arrow_forwardSome strains of Escherichia coli bacteria have acquired the ability to produce the harmful Shiga toxin, normally produced by Shigella dysenteriae. Which statements best explain why this occurs? Mark all that apply. A. conjugation between two different species of bacteria allowed both strains of bacteria to express a virulence factor B. transformation resulted in expression of a virulence factor C. horizontal gene transfer between different species of bacteria D. transduction of a specific gene resulted in expression of new traitsarrow_forward
- Differentiate horizontal and vertical gene transfer. Compare the mechanisms of genetic recombination in bacteria (define/contrast: transformation, conjugation, and transduction). Characterize the 4 different cell types involved in conjugation.arrow_forwardWrite and detail a complete all encompassing definition of a gene. Consider the morphology, physiology, ecological, behavioral, etc. aspects of the gene. Discuss each part of the gene listed in your definition. Is your definition of the gene as determined through complementation, recombination, and mutation for prokaryotes directly applicable to eucaryotes? Why or why not? What additional information must be taken into account?arrow_forward(explain how the bands move threw the gel?)arrow_forward
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