Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 10MCQ
A gene that hybridizes with mRNA.
- a. antisense
- b. clone
- c. library
- d. Southern blot
- e. vector
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Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Which of the following is not an example of a point mutation? a. nonsense b. sense c. antisense d. missensearrow_forwardWe have isolated a mutant that changes transcription of many mRNAs, we can use to determine if there are changes to mRNA levels A. microarrays/Sequencing technologies B. Sanger sequencing C. western blot D. all of the abovearrow_forwardWhich genes can be transferred by all three methods of horizontal gene transfer? a. capsule production b. toxin production c. F factord. drug resistancearrow_forward
- Which of the following methods use(s) a labeled nucleic acidprobe, such as a labeled fragment of DNA?a. Site-directed mutagenesis c. Western blottingb. Northern blotting d. Both a and barrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forwardSome strains of Escherichia coli bacteria have acquired the ability to produce the harmful Shiga toxin, normally produced by Shigella dysenteriae. Which statements best explain why this occurs? Mark all that apply. A. conjugation between two different species of bacteria allowed both strains of bacteria to express a virulence factor B. transformation resulted in expression of a virulence factor C. horizontal gene transfer between different species of bacteria D. transduction of a specific gene resulted in expression of new traitsarrow_forward
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- Discuss the process and give example on the following: a. Genome Project-write b. Mitochondrial DNA 'Spring Cleaning' c. Micro-encapsulation d. Specific Drug Targeting Answer a,b,c,darrow_forwardThe function of CRISPR gene cluster in Streptococcus pyogenes is to Select one or more: a. destroy phage DNA in the bacteria. O b. protect phage DNA in the bacteria. Oc. repair the damaged DNA in its genome. O d. delete the specific genes in its genome.arrow_forwardAn experimental drug therapy to treat patients with antibiotic-resistant bacteria involves introduction of a highly specific bacteriophage to the infected patient's bloodstream. Which of the following bacteriophage types would be the LEAST useful for this therapy? a. a lytic bacteriophage b. An enveloped virus c. An RNA virus d. a lysogenic bacteriophagearrow_forward
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