Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 2MCQ
The DNA probe, 3′-GGCTTA, will hybridize with which of the following?
- a. 5′-CCGUUA
- b. 5′-CCGAAT
- c. 5′-GGCTTA
- d. 3′-CCGAAT
- e. 3′-GGCAAU
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The restriction enzyme PbtIX leaves the site CTGCA|G between the A and G as shown. (As always, the DNA sequence shown is 5'->3'). This leaves a
a. 5' overhang
b. 5' overhang on one end of the cut, and 3' overhang on the other end of the cut
c. blunt end
d. 3' overhang
e. none of the above
HindIII is a restriction endonuclease that recognizes a palindrome sequence. Predict which one is the HindIII recognition site:
a.
AAGGTT
b.
AAGCTT
c.
AGCCGA
d.
AGCGCA
e.
AGCAGC
Which of the following is a sequence most likely to be recognized by restriction enzymes?
a. 5' GATTAATC 3' - double-strand form
b. 5' ATG 3' - double-strand form
c. 5' AUGCGCAU 3' - double-strand form
d. NH3-met-trp-val- COOH
e. none of the above
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- The picture below depicts an aberration in the process of genetic coding. Which of the following terms best describes this occasional occurrence in genetic material? see photo attached a. Chromosomal aberration b. Mutation c. CRISPR d. ZNFarrow_forwardWhich of the following is not a DNA sequence? a. TAG b. AUGAUUCT d. AAAAAAAAAA e. CGGarrow_forwardWhich DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides? a.DNA-Pol I b.DNA-Pol II c.DNA-Pol Ill d.DNA-Pol IVarrow_forward
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- Please explain right answerarrow_forward_____________ are sequences of identical DNA when it's read from the 5' to 3' direction on one strand and the 5' to 3' direction on the other strand. a. Palindromes b. snRNPs c. RFLPs d. Restriction fragments e. Restriction endonucleasesarrow_forwardThe function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forward
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