Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 9, Problem 7MCQ
Pieces of human DNA stored in yeast cells.
- a. antisense
- b. clone
- c. library
- d. Southern blot
- e. vector
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
In the 1953 study by Hershey and Chase
A. 32P was found in supernatants after T2 infection
B. 35S was found in bacterial pellets after T2 infection
C. E. coli genomes were inherited after T2 infection
D. RNA was excluded as THE heritable material
E. T2 coats were removed with a blender after initial phage binding
Some strains of Escherichia coli bacteria have acquired the ability to produce the harmful Shiga toxin, normally produced by Shigella dysenteriae. Which statements best explain why this occurs? Mark all that apply.
A. conjugation between two different species of bacteria allowed both strains of bacteria to express a virulence factor
B. transformation resulted in expression of a virulence factor
C. horizontal gene transfer between different species of bacteria
D. transduction of a specific gene resulted in expression of new traits
Which is the BEST technique to use to answer this question?
You wish to determine whether a point mutation is present in the sunscreen gene.
a. Westem blot.
b. Northern blot .
c. Sanger DNA sequencing
d. In situ hybridization.
Part B. Which is the BEST technique to use to answer this question? You wish to determine where Sunshine protein is translated in a ladybug embryo.
a. Immunofluorescencez
b. Western blot .
c. RT-PCR.
d. Northern blot.
e. in situ hybridization.
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
Additional Science Textbook Solutions
Find more solutions based on key concepts
Why is it necessary to be in a pressurized cabin when flying at 30,000 feet?
Anatomy & Physiology
6. How can you use the features found in each chapter?
Human Anatomy & Physiology (2nd Edition)
Some people consider Pasteur or Koch to be the Father of Microbiology, rather than Leeuwenhoek. Why might they ...
Microbiology with Diseases by Body System (5th Edition)
6. How can you use the features found in each chapter?
Human Anatomy & Physiology
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Souther blot is used to detect ______________whereas Nothern blot is used to detect ________________. A. RNA, proteins B. RNA, DNA C. DNA, Proteinsarrow_forwardDefine the following terms: a. lytic cycle b. prophage c. transduction d. lysogeny e. nuclocapsidarrow_forwardWhich Class I element has likely experienced the greatest accumulation of mutations compared to its retrovirus ancestor, as its DNA sequence is the most divergent? A. LINES B. transposons C. Ac/Ds elements D. SINES E. LTR retrotransposonsarrow_forward
- Which of the following cannot usually be inserted into a vector to form a recombinant DNA? A. PCR product of a eukaryotic coding sequence B. Lac operon operator region DNA sequence C. Reverse transcriptase gene D. E. coliarrow_forwardIf the HIV did not have a reverse transcriptase enzyme, then which of the following would apply? Select one: a. The virus can make use of the reverse transcriptase enzyme from the host cell to convert its RNA into DNA b. Viral particles will be produced without reverse transcriptase enzyme since the original HIV is an RNA virus and needs to assemble RNA ant coat proteins together c. The virus will manufacture its proteins from its RNA using translation and ribosomes from host cell d. Provirus will not be formed and assembly of viral particles will never occur e. The virus will not be able to recognise the host cell and therefore entry into the cell is blockedarrow_forwardIn the phage titer experiment, why did you plate multiple dilutions? a. so that you should have a countable plate b. to have duplicates to count c. As a control d. to be certain the E. coli was not previously infectedarrow_forward
- 96) The RT enzyme: a. The output is dsDNA. b. The enzyme works in the nucleus in case of the HIV virus. c. the template of this enzyme is double stranded RNA d. Found in the capsid of FMD virusarrow_forwardafter a successful infection by a viral particle, it integrates its genome into the host DNA. which term best describe the change of host genome? a. none b.silent mutation c.missense mutation d.frame shift mutationarrow_forward91) The NDV virus a. The mesogenic ND is less virulent than lentogenic than NDV b. The antigenomic copy is the template for both making genome and making subgenus c. The RNA polymerase is only made in the first round of translation d. The Fusion protein is the virulent proteinarrow_forward
- What are the types of in vitro virus cultivation systems (Choose all that applies) A. Animal cell culture B. Bird embryos (eggs) C. Using live animals D. Special media made to grow viruses.arrow_forwardwhich of the following can be used to stimulate an organism's immune system? A. Restriction Enzymes B. DNA Ligase C. Sticky Ends D. Monoclonal Antibodiesarrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
DNA Use In Forensic Science; Author: DeBacco University;https://www.youtube.com/watch?v=2YIG3lUP-74;License: Standard YouTube License, CC-BY
Analysing forensic evidence | The Laboratory; Author: Wellcome Collection;https://www.youtube.com/watch?v=68Y-OamcTJ8;License: Standard YouTube License, CC-BY