Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 9, Problem 9R
How does RNAi “silence” a gene?
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Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Amino Acid Coclow TABle 3' Gly Phe Leu (G) (F) (L) 3- Val (V) Arg (R) Ser (S) Ala (A) Lys (K) CAG G Glu Asp (E) (D) Ser (S) CCCAGUCAGUCAGUCAG 0204 C U A G C Asn (N) G 4 A AGU C GU (5) AC C UGA A G5 C CUGACUGACUGACUGAC Thr (T) Met (M) lle £€ (1) U 4 G Tyr Σε (Y) U Cys (C) C A G Trp (W) 3' U C A Leu בוט His Pro (P) ££ (H) Gin (Q) Arg 흐름 (R) (L) Start Stop 8. Transcription and Translation Practice: (Video 10-1 and 10-2) A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense DNA strand and label the 5' and 3' ends. B. Use the antisense strand that you create in part A as a template to create the mRNA transcript of the gene and label the 5' and 3' ends. C. Translate the mRNA you produced in part B into the polypeptide sequence making sure to follow all the rules of translation. 5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand) 4arrow_forwardWhat is the structure and function of Eukaryotic cells, including their organelles? How are Eukaryotic cells different than Prokaryotic cells, in terms of evolution which form of the cell might have came first? How do Eukaryotic cells become malignant (cancerous)?arrow_forwardWhat are the roles of DNA and proteins inside of the cell? What are the building blocks or molecular components of the DNA and proteins? How are proteins produced within the cell? What connection is there between DNA, proteins, and the cell cycle? What is the relationship between DNA, proteins, and Cancer?arrow_forward
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