Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 8R
You are attempting to insert a gene for saltwater tolerance into a plant by using the Ti plasmid. In addition to the desired gene, you add a gene for tetracycline resistance (tetR) to the plasmid. What is the purpose of the tetR gene?
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Why is it important to include a selective marker gene on a plasmid?
a) So that you can select cells containing the plasmid after transformation.
b) So that you can select where the plasmid inserts into the genome.
c) So that you can select the recombinant gene you want to add to the plasmid.
d) So that you can select when the recombinant gene on your plasmid is
expressed.
Why is it important to include a selective marker gene on a plasmid?
a) So that you can select cells containing the plasmid after transformation.
b) So that you can select where the plasmid inserts into the genome.
Oc) So that you can select the recombinant gene you want to add to the plasmid.
d) So that you can select when the recombinant gene on your plasmid is
expressed.
1)What is plasmid?
2) How do you explain the selection of plasmid for carrying the desired gene?
3) Follow the steps of human insulin production
4) Make a diagram for the production of growth hormone
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- You want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How should this plasmid vector be constructed? b) Also describe the function of the components.arrow_forwardWhat do you mean by vector plasmid?arrow_forwardWhen a 5-kb circular plasmid is digested with a restriction enzyme that has three recognition sites on the plasmid, how many bands can be visualized on an agarose gel? a)1 b)2 c)5 d)3arrow_forward
- Why do we need a plasmid to transform a bacteria? What are the essential components of a plasmid and explain their functions. What is the difference between a Plasmid and a Vector?arrow_forwardYou have set up a recombinant DNA experiment using the plasmid PBR322 as the vector (see plasmid below). You use the BamHI restriction site on the plasmid to insert the target DNA. The plasmid is then used to transform E.coli colls Is the following statement True or False? Growth of the transformed cells on agar containing both ampicillin and tetracycline will eliminate any cells that do not contain a plasmid. Clal Hindlll EcoRI Pvul BamHI Pstl amp tet PBR322 -Sall ori rop Pvull True Falsearrow_forwardList the major steps that would be required to clone the human insulin gene into a plasmid and to identify bacterial colonies that contain the plasmid with the insulin gene inserted. Be sure to include a suggestion for how to select colonies with a plasmid and how to distinguish plasmids with and without the insulin gene insertedarrow_forward
- EDTA weakens the cell wall by removing ions that help hold it together while glucose prevents premature cell lysis due to osmosis in cells with weakened cell walls. You have made a batch of resuspension solution to isolate plasmid DNA, but you forgot to add EDTA. What do you think will happen when you try to perform a plasmid isolation procedure with this reagent? Why?arrow_forwardIf we were to examine a strain with the F plasmid inserted into the same site of the bacterial chromosome, but in the reverse orientation: a) What would the order of gene transfer be? Include all of the genetic markers including the amino acid and nucleic acid metabolism genes and streptomycin resistance. b) What cell types would be able to grow on the NA vs ECM media types? Be sure to include the genotypes of the cells that would grow. Remember that NA provides all nutrients the bacteria needs + no antibiotic and HCM = minimal medium + glucose + has streptomycin antibiotic c) Would we still be able to perform our mapping? Why or why not? (Hint: refer to part b above)arrow_forwardA plasmid provides its host with kanamycin resistance. What will happen to the plasmid if you culture its host for many generations in the medium that does not contain kanamycin?arrow_forward
- A student wishes to transform some E. coli with a plasmid to “knock-out” (ie. “silence” or prevent) the activity of Gene X, a gene important in the metabolism of Nutrient X. If Gene X is expressed, it can metabolize Nutrient X, contained within the agar plates, to produce neon orange colonies. To perform this transformation, the student knows that a plasmid must be used. You can assume that the materials this student has to perform their transformation are the same as those you had to complete lab #3. With the above in mind, please answer the following a) What is a plasmid and why does this student need to use one to perform their transformati b) How should the student build their plasmid? Identify and briefly describe three key components they should include in their plasmid. c) Explain how the student can make their bacterial cells competent to receive their plasmid DNA. (3 pts.)arrow_forwardWhat is plasmid? What is the purpose of plasmid and how is it important?arrow_forwardBesides their role in genetic engineering, what else do plasmids can plasmids be used for? How are restriction enzymes used in genetic engineering?arrow_forward
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