Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 1MCQ
Restriction enzymes were first discovered with the observation that
- a. DNA is restricted to the nucleus.
- b. phage DNA is destroyed in a host cell.
- c. foreign DNA is kept out of a cell.
- d. foreign DNA is restricted to the cytoplasm.
- e. all of the above
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The other options are:
a. RNA cannot be digested by restriction enzymes
b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion.
c. RNA is single stranded and DNA is double stranded
Which of the following is a function of a restriction endonuclease?
a.
It cleaves a DNA molecule at a specific site.
b.
It cleaves a DNA molecule at random locations.
c.
It removes specific codons from an mRNA molecule.
d.
It removes specific exons from an mRNA molecule.
Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- In nucleotide excision repair in E. coli, the function of the UvrA/UvrB complex is toa. detect DNA damage.b. make cuts on both sides of the damage.c. remove the damaged piece of DNA.d. replace the damaged DNA with undamaged DNA.arrow_forwardRestriction enzymes found in bacterial cells are ordinarily useda. during DNA replication.b. to degrade the bacterial cell’s DNA.c. to degrade viral DNA that enters the cell.d. to attach pieces of DNA together.arrow_forwardRestriction enzymes (type II) bind to their recognition site and A. begin elongation from it B. methylaet it C. cut inside it D. ligate itarrow_forward
- Features of restriction enzymes include the following, except __________ . a. They are produced by both prokaryotes and eukaryotesb. may be type IIc. make single and double stranded breaks in DNAd. operate at a single temperaturee. Are used in nature to destroy bacteriophage DNAarrow_forwardOne would be correct in assuming that the restriction sites present in the multiple cloning site of the pUC18 plasmid will a. be the only instance of that restriction site in the plasmid b. appear at least once in another location in the vector. c. only include 4 bp restriction enzyme recognition sites. d. only be recognition sites that produce blunt ends when cleaved by the appropriate enzyme.arrow_forwardWhat is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbonesarrow_forward
- The recognition site of some restriction enzymes are shown. Which will produce sticky ends? A. EcoRI G↓AATTC. B. SmaI CCC↓GGG. C. XhoI C↓TCGAG. D. Both EcoRI and XhoI. E. All the enzymes shown will leave sticky ends.arrow_forwardOne would be correct in assuming that the restriction sites present in the multiplecloning site of the pUC18 plasmid willarrow_forwardMatch items in Column A with most related ones in B. Write the letters on the space provided.arrow_forward
- Certain restriction endonucleases produce cohesive (sticky) ends. This means that they: a. stick tightly to the ends of the DNA they have cut. b. cut both DNA strands at the same base pair. c. make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding. d. cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content. e. cut in regions of high AT content, leaving ends that can form more hydrogen bonds than ends of high GC content.arrow_forwardA genetic map provides a. the sequence of the DNA in a genome. b. the relative position of genes on chromosomes. c. the location of sites of restriction enzyme cleavage in a known sequence of DNA. d. the banding pattern of a chromosome.arrow_forwardWhich of the following cannot usually be inserted into a vector to form a recombinant DNA? A. PCR product of a eukaryotic coding sequence B. Lac operon operator region DNA sequence C. Reverse transcriptase gene D. E. coliarrow_forward
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