Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 4MCQ
The following enzymes are used to make cDNA. What is the second enzyme used to make cDNA?
- a. reverse transcriptase
- b. ribozyme
- c. RNA polymerase
- d. DNA polymerase
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Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Some antibiotic drugs fight infection by interfering with DNA replication, transcription, or translation in bacteria. Indicate whether each of the following antibiotic drug effects is on replication, transcription, or translation. HINT Each answer (replication, transcription, and translation) is used only once for the following: a. Rifampin binds to bacterial RNA polymerase. b. Streptomycin binds bacterial ribosomes, disabling them. c. Quinolone blocks an enzyme that prevents bacterial DNA from unwinding.arrow_forwardWhich of the following is synthesized from a DNA template? (Select one) a. Transducer RNA b. ribosomal RNA c. amino acids d. translation RNAarrow_forwardWhich of the following molecules helps relieve the tension of unwinding parental DNA strands, by breaking DNA and rejoining it before it is replicated? a. Primase b. Single-stranded binding proteins c. Helicase d. Topoisomerasearrow_forward
- Which process does NOT use a specific nucleotide sequence for activity? please explain the answer a.replication initiation b.DNA helicase unwinding c.transcriptional termination d.translational terminationarrow_forwardThe polymerase chain reaction requires the following except A. primers complementary to the ends of the sequence to be amplified B. carefully controlled temperature conditions C. cycles of denaturation, annealing, and extension D. RNA polymerase to synthesize RNA primersarrow_forwardRead the images given before answering the question below. Identify the two common diseases that result from mutations in gene A?arrow_forward
- The smallpox virus, which actually has a fairly large genome size for a virus (about 190,000 base pairs), must use which of the following enzymes to replicate its nucleic acid? A. DNA-dependent-DNA-polymerase B. RNA-dependent-DNA-polymerase C. DNA-dependent-RNA-polymerase D. RNA-dependent-RNA-polymerase E. reverse transcriptasearrow_forwardDNA damage by uv light creates pyrimidine dimers between adjacent pyrimidines on the same strand. The cellular mechanism used to repair these dimers is : A. Mismatch repair B. Proofreading C. Nucleotide Excision Repair D. Single- base excisionarrow_forwardWhich of the following enzymes is responsible for ensuring that genetic material at the end of the chromosome is not lost during DNA replication? a. Helicase b. DNA Polymerase c. Telomerase d. DNA Ligase e. Primasearrow_forward
- Which of the following enzymes adds a new amino acid to the growing chain of a protein during protein synthesis? a. aminoacyl-tRNA synthetase b. peptidyl synthetase c. peptidyl transferase d. ribosomal synthetasearrow_forwardFor each function, please name the enzyme or ribozyme that performs the function. 1. Transcribes RNA from a DNA template Synthesizes a complementary DNA strand from an RNA template Replicates DNA from a DNA template Replicates RNA from an RNA templatearrow_forwardEnzymes or proteins involved in DNA repair include a. RAD52 b. RuvA c. UvrA d. MAPKarrow_forward
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