Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 4MCQ
The following enzymes are used to make cDNA. What is the second enzyme used to make cDNA?
- a. reverse transcriptase
- b. ribozyme
- c. RNA polymerase
- d. DNA polymerase
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Match the following polymerases with their function.
+ DNA Polymerase III
DNA polymerase I
+ RNA polymerase
+ RNA polymerase II
A. Polymerase responsible for proofreading
DNA
B. Polymerase responsible for making
prokaryotic mRNA
C. Polymerase responsible for making
eukaryotic MRNA
D. Polymerase responsible for making
prokaryotic DNA
Define the following terms: a. processivity b. replisome c. exonuclease d. DNA ligase e. replicon
Which enzyme is used in Sanger sequencing reactions?
O A. DNA polymerase
O B. S1 endonuclease
O C. ligase
O D. reverse transcriptase
O E. RNA-dependent DNA polymerase
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Some antibiotic drugs fight infection by interfering with DNA replication, transcription, or translation in bacteria. Indicate whether each of the following antibiotic drug effects is on replication, transcription, or translation. HINT Each answer (replication, transcription, and translation) is used only once for the following: a. Rifampin binds to bacterial RNA polymerase. b. Streptomycin binds bacterial ribosomes, disabling them. c. Quinolone blocks an enzyme that prevents bacterial DNA from unwinding.arrow_forwardWhich of the following molecules helps relieve the tension of unwinding parental DNA strands, by breaking DNA and rejoining it before it is replicated? a. Primase b. Single-stranded binding proteins c. Helicase d. Topoisomerasearrow_forwardHow many subunits does the E. coli DNA polymerase I have? Select one: O a. 5 O b. 10 O c. O d. 3arrow_forward
- The polymerase chain reaction requires the following except A. primers complementary to the ends of the sequence to be amplified B. carefully controlled temperature conditions C. cycles of denaturation, annealing, and extension D. RNA polymerase to synthesize RNA primersarrow_forwardDNA damage by uv light creates pyrimidine dimers between adjacent pyrimidines on the same strand. The cellular mechanism used to repair these dimers is : A. Mismatch repair B. Proofreading C. Nucleotide Excision Repair D. Single- base excisionarrow_forwardWhich of the following enzymes adds a new amino acid to the growing chain of a protein during protein synthesis? a. aminoacyl-tRNA synthetase b. peptidyl synthetase c. peptidyl transferase d. ribosomal synthetasearrow_forward
- Assume that a mutation occurs in the gene that encodes each of the following RNA polymerases. Match the mutation with its possible effects by placing the correct letter or letters in the blanks below. There may be more than one effect for each mutated polymerase. A mutation in the gene that codes for E RNA polymerase I RNA polymerase II RNA polymerase III Possibleeffects a. tRNA is not synthesized b. Some ribosomal RNA is not synthesized c. Ribosomal RNA is not processed d. pre-mRNA is not processed e. Some mRNA molecules are not degraded f. pre-mRNA is not synthesizedarrow_forwardEnzymes or proteins involved in DNA repair include a. RAD52 b. RuvA c. UvrA d. MAPKarrow_forwardThe following enzymes cannot catalyze the synthesis of DNA A.DNA polymerase B.DNA gyrase C.DNA ligase D.Helicasearrow_forward
- Eukaryotes such as humans have linear chromosomes. In order to signal the end of DNA replication, there is a large repetitive sequence of DNA called a telomere. The telomere region of the DNA signals a process called a. detachment b. termination c. elongation d. transcriptionarrow_forwardA mutant DNA strand was transcribed then translated to proteins. a. What is the protein product of the mutant DNA strand? The sequence of the mutant strand is shown below: 5'-TGCCATAACTGTTCGTACTGGCAAATTGCC-3' 3'-ACGGTATTGACAAGCATGACCGTTTAACGG-5' b. The mutation altered the sequence of the wild type template DNA such that a degenerate codon for a basic amino acid in the wild type was converted to a non-degenerate codon resulting in the sequence for the mutant strand shown. What was the original amino acid? c. Compare the charges and pl of the mutant peptide and the normal (wild- type) peptide at physiological pH?arrow_forwardWhich statement about Okazaki fragments is true? Select one: a. DNA polymerase doesn’t need a primer to build these fragments b. They act as a primer that initiates DNA replication. c. They correct errors made during earlier phases of DNA replication. d. They are necessary because DNA polymerase can only build DNA in the 5’ to 3’ direction, so for one of the strands at each fork, the DNA polymerase can only buildaway from the fork. e. They prevent the ends of chromosomes from shortening with every replication.arrow_forward
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