Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 9, Problem 6MCQ

You have a small gene that you want replicated by PCR. You add radioactively labeled nucleotides to the PCR thermal cycler. After three replication cycles, what percentage of the DNA single strands are radioactively labeled?

  1. a. 0%
  2. b. 12.5%
  3. c. 50%
  4. d. 87.5%
  5. e. 100%
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PCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur. A. DNA template B. Thermo stable DNA polymerase C. Two primers D. Type I endonucleases
Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)?   a. PCR requires a copy of RNA that serves as a template   b. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA   c. Annealing requires cooler temperatures than denaturation   d. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA   e. PCR is a quick way of using minute quantities of DNA to create millions of copies
A double stranded DNA molecule is shown below.  The same DNA is shown below with the two strands separated.  A series of potential PCR primers (labeled 1, 2, 3 and 4) that are complementary to the template DNA are shown. Which set(s) of PCR primers would allow a researcher to amplify the sequences beginning around position 10 and ending around 40? A. Primer 2 and Primer 3 B. Primer 2 and  Primer 4 C. Primer 1 and Primer 4 D. Primer 1 and Primer 3
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