Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 9, Problem 6MCQ
You have a small gene that you want replicated by PCR. You add radioactively labeled
- a. 0%
- b. 12.5%
- c. 50%
- d. 87.5%
- e. 100%
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Students have asked these similar questions
PCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur.
A. DNA template
B. Thermo stable DNA polymerase
C. Two primers
D. Type I endonucleases
Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)?
a.
PCR requires a copy of RNA that serves as a template
b.
Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA
c.
Annealing requires cooler temperatures than denaturation
d.
Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA
e.
PCR is a quick way of using minute quantities of DNA to create millions of copies
A double stranded DNA molecule is shown below. The same DNA is shown below with the two strands separated. A series of potential PCR primers (labeled 1, 2, 3 and 4) that are complementary to the template DNA are shown.
Which set(s) of PCR primers would allow a researcher to amplify the sequences beginning around position 10 and ending around 40?
A. Primer 2 and Primer 3
B. Primer 2 and Primer 4
C. Primer 1 and Primer 4
D. Primer 1 and Primer 3
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- All are true about PCR except A. Automated PCR machine called a thermal cycler B. A thermostable DNA polymerase is required C. Primer extension occurs at 72˚C D. Annealing involves binding of primer between 90-98˚Carrow_forwardIf a PCR is started using 10 pieces of template DNA, how many pieces of DNA would there be after 10 cycles? a. About 100 b. About 1000 c. About 10,000 d. About 1010arrow_forwardA linear piece of DNA is cut by two restriction enzymes. One restriction enzyme will cut the target DNA once. The other restriction enzyme will cut the target DNA twice. How many fragments of DNA should result from this reaction? a. 3 fragments of DNA b. 4 fragments of DNA c. 5 fragments of DNA d. 6 fragments of DNA e. None of the abovearrow_forward
- What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one personarrow_forwardThe image below represents the PCR results after electrophoresis on an agarose gel. One band is labelled as (i) and another is labelled as (ii). Which of the following descriptions below best describes the band labelled as (i)? a. Relaxed DNA b. Primers c. RNA d. Amplicon e. Digested DNA fragment f. None of the abovearrow_forwardWhat would be the outcome if the primers used in a polymerase chain reaction have lower GC content (<40 %), shorter, and more variable than the intended oligonucleotide sequence? a.The PCR reaction will cease after the first cycle b.The reaction will yield a mixture of non-specific products. c.All of these d.The PCR reaction will not start. e.The reaction will yield a single short PCR product.arrow_forward
- Match the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.arrow_forwardAfter running PCR on your raw milk colony, you run your product on an agarose gel and see multiple bands coming from a single sample. Assuming there was no contamination in the set up of your PCR reaction, what could have happened? a. the primers were not specific enough b. the PCR reaction was run for too long c. you forgot to add primers d. the PCR annealing temperature was too high e. the master mix lacked Taq enzyme f. both a and darrow_forwardWould it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting. c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.arrow_forward
- For DNA amplification using PCR to occur, which of the following are needed? A. DNA primers B. thermo stable DNA polymerase C. Helicase D. Choices A and Barrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forwardPolymerase Chain Reaction, or PCR, can Group of answer choices A. target a specific region of DNA and cut it out of the rest of the genetic material for further analysis. B. copy the number of copies of a selected region of DNA linearly. C. increase the number of copies of a selected region of DNA exponentially. D. copy the entire genome at least a dozen times.arrow_forward
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