Chapter 6, Problem 68TQ

Summary Introduction

To review:

The activity of fumarate and malate in the citric acid cycle by Michaelis–Mentenconstant(K_m) and turnover number (k_cat), when ${\text{K}}_{\text{m}}=\text{5}\times {\text{10}}^{\text{-6}}\text{M}$ and ${\text{K}}_{\text{m}}=\text{5}\times {\text{10}}^{\text{-6}}\text{M}$ for fumarate; and ${\text{K}}_{\text{m}}=\text{2}\text{.5}\times {\text{10}}^{\text{-5}}\text{M}$ and ${\text{k}}_{\text{cat}}=\text{9}\times {\text{10}}^{\text{2}}{\text{s}}^{\text{-1}}$ for malate.

Introduction:

The binding affinity of the enzyme for the substrate is determined by ${\text{K}}_{\text{m}}$ .The number of substrate molecule converted into product per unit time is determined by ${\text{k}}_{\text{cat}}$. ${\text{K}}_{\text{m}}$ and k_cat are used to determine the specificity constant of the enzyme.