Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 4, Problem 28P

Yeast are single-celled eukaryotic organisms that grow in culture as either haploids or diploids. Diploid yeast are generated when two haploid strains fuse together. Seven haploid strains of yeast exhibit similar growth habit: At 25°C, each strain grows normally, but at 37°C , they show different growth capabilities. The table below displays the growth pattern.

Chapter 4, Problem 28P, 28. Yeast are single-celled eukaryotic organisms that grow in culture as either haploids or , example  1

Describe the nature of the mutation affecting each of these mutant yeast strains. Explain why strains B and G display different growth habit at 37°C than the other strains. b. Each of the mutant pairs of haploid yeast is fused, and the resulting diploids are tested for their ability to grow at 37°C . The results of the growth experiment are shown below.

Chapter 4, Problem 28P, 28. Yeast are single-celled eukaryotic organisms that grow in culture as either haploids or , example  2

How many different genes are mutated among these seven yeast strains? Identify the strains that represent each gene mutation.

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You are interested in studying resistance to heavy metals and have selected the yeast Saccharomyces cerevisea to conduct your studies. You have recovered a deletion mutant that does not tolerate high concentrations of zinc (grows poorly in zinc containing media ) and have designated the mutant pgz-1 (for poor growth in zinc ). (a) What is the advantage to the type of mutant used in this work? What class of mutagen was likely use to generate pgz-1? ( b) Do you expect the PGZ gene to be expressed in your mutant? Explain.
Three different chitin synthase genes control chitin synthesis in S. cerevisiae. Discuss what will happen to the budding yeast if a mutation occurs in each of the genes below: CHSI CHSII CHSIII
What is the correct answer, and why?

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Genetic Analysis: An Integrated Approach (3rd Edition)

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