A restriction enzyme known as Bg/II cuts after Adenine near to 5 ' in the given DNA. The complementary 3 ' to 5 ' strand should be drawn for the above DNA and shown where it is cut by the same enzyme. Concept Introduction: Restriction endonuclease is a restriction enzyme that cuts double stranded or single stranded DNA at specific recognition nucleotide sequences (mostly palindromic) known as restriction sites. Palindromic sequence is the mirror like palindrome in which the same forward and backwards are on a single strand of DNA strand. Example for palindrome sequence: 5 ' − G − T − T − A − A − C − 3 ' 3 ' − C − A − A − T − T − G − 5 ' The base sequences that most restriction endonuclease recognizes are palindromes.

Question

Want to see the full answer?

Answer 1

Question

Chapter 27.4, Problem 27.3P

Interpretation Introduction

Interpretation:

A restriction enzyme known as Bg/II cuts after Adenine near to 5' in the given DNA. The complementary 3' to 5' strand should be drawn for the above DNA and shown where it is cut by the same enzyme.

Concept Introduction:

Restriction endonuclease is a restriction enzyme that cuts double stranded or single stranded DNA at specific recognition nucleotide sequences (mostly palindromic) known as restriction sites.

Palindromic sequence is the mirror like palindrome in which the same forward and backwards are on a single strand of DNA strand.
Example for palindrome sequence:

5'− G−T−T −A−A−C−3'3'−C−A−A−T−T−G−5'

The base sequences that most restriction endonuclease recognizes are palindromes.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Discuss the differences between eukaryotic and prokaryotic cells with regards to their genetic materials. Including in your discussion the structure and organisation of genetic material, as well as any implications these differences may have on cellular functions and evolution. Rubric Understanding of genetic material differences (provides a comprehensive and accurate explanation of the differences between eukaryotic and prokaryotic cells in terms of their genetic material, including the structure, organisation and function of genetic material in each cell type. Demonstrates a thorough understanding of the topic) Analysis of implications (analyze the differences between eukaryotic and prokaryotic cells in terms of their genetic material with depth and insight, discussing the implications of these differences on cellular functions and evolution. Provides specific examples and explanations.)

When was the dihydropyridine calcium channel blocker isradipine first patented and by whom? Please provide information on the origin and history of isradipine and who owns it/manufactures it.

9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and 14.2 kDa, a) Figure 4: indicate where each of the measurements were taken and label as in II.6. figure 2 above. b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f. values for the MW standards, plot the relative mobility versus the log of the mass for the standards, and use the best fit straight line to determine the molecular weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be scaled up appropriately if your gel did not develop properly) dye MW Whey Peak 1 Peak 2