Fundamentals of General, Organic, and Biological Chemistry (8th Edition)
8th Edition
ISBN: 9780134015187
Author: John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Question
Chapter 27.5, Problem 27.5CIAP
Interpretation Introduction
Interpretation:
The five basic steps of DNA fingerprinting using the RFLP method has to be listed and the reason for usage of PCR method in crime investigations has to be identified.
Concept Introduction:
RFLP method:
RFLP is abbreviated as restriction fragment length polymorphism; it is technique which raises the variations in homologous DNA sequences. This technique has cutting the particular region of DNA with known variability with the restriction enzymes then separating the DNA pieces by gel electrophoresis.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
State the five basic steps of DNA fingerprinting using the RFLP method. Why do you think the PCR method is of more use in crime scene investigations?
Discuss the principle of DNA fingerprinting. How would you use the technique to find out a criminal in forensic studies ? Explain.
What is DNA fingerprinting? Mention its application.
Chapter 27 Solutions
Fundamentals of General, Organic, and Biological Chemistry (8th Edition)
Ch. 27.1 - Decode the following sequence of letters to find...Ch. 27.3 - Prob. 27.1CIAPCh. 27.3 - Prob. 27.2CIAPCh. 27.3 - Prob. 27.3CIAPCh. 27.3 - Prob. 27.2KCPCh. 27.4 - Prob. 27.3PCh. 27.4 - A restriction enzyme known as EcoRI cuts DNA in...Ch. 27.4 - Prob. 27.5PCh. 27.5 - Classify the following activities according to the...Ch. 27.5 - Prob. 27.4CIAP
Ch. 27.5 - Prob. 27.5CIAPCh. 27.5 - Prob. 27.6CIAPCh. 27 - What steps are necessary in the mapping of the...Ch. 27 - Prob. 27.8UKCCh. 27 - List the four types of noncoding DNA (see Section...Ch. 27 - In general, what are the differences between...Ch. 27 - What is recombinant DNA? How can it be used to...Ch. 27 - Identify some major potential benefits of the...Ch. 27 - Prob. 27.13APCh. 27 - Prob. 27.14APCh. 27 - Prob. 27.15APCh. 27 - Prob. 27.16APCh. 27 - Prob. 27.17APCh. 27 - Prob. 27.18APCh. 27 - Prob. 27.19APCh. 27 - You may have heard of Dolly, the cloned sheep...Ch. 27 - Prob. 27.21APCh. 27 - Prob. 27.22APCh. 27 - What is the role of the enzyme telomerase? In what...Ch. 27 - Prob. 27.24APCh. 27 - Prob. 27.25APCh. 27 - Prob. 27.26APCh. 27 - Prob. 27.27APCh. 27 - What is a SNP?Ch. 27 - How are SNPs linked to traits in individual human...Ch. 27 - List some potential biological effects of SNPs.Ch. 27 - Prob. 27.31APCh. 27 - Prob. 27.32APCh. 27 - Prob. 27.33APCh. 27 - Prob. 27.34APCh. 27 - Prob. 27.35APCh. 27 - Prob. 27.36APCh. 27 - Prob. 27.37APCh. 27 - Prob. 27.38APCh. 27 - In the formation of recombinant DNA. a restriction...Ch. 27 - Give the sequence of unpaired bases that would be...Ch. 27 - Are the following base sequences sticky or not...Ch. 27 - Prob. 27.42APCh. 27 - Prob. 27.43APCh. 27 - Provide two examples of genetically engineered...Ch. 27 - Prob. 27.45APCh. 27 - Why is the field of bioethics so important in...Ch. 27 - Prob. 27.47CPCh. 27 - Prob. 27.48CPCh. 27 - Prob. 27.49CPCh. 27 - Prob. 27.50CPCh. 27 - What is a restriction endonuclease?Ch. 27 - Prob. 27.52CPCh. 27 - Prob. 27.53GPCh. 27 - One of the most actively pursued areas in genomics...Ch. 27 - Prob. 27.55GP
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- What is DNA phenotyping, and how do law enforcement agencies use this profiling method?arrow_forwardBrenda is a junior student in the biomedical program at her school. She is starting the PCR genetic testing lab activity. She is about to obtain her DNA sample but doesn’t want like the taste of NaCl solution. Her friend, Mark, let her use some of his DNA. What laboratory tule did the students break? A. Obtaining and handling DNA sample without wearing googles or gloves B. Improper use of human DNA samples C. Violating Patient Confidentiality D. Disposing of bio hazardous material in a regular trasharrow_forwardWhat are the steps /procedure in DNA fingerprinting?arrow_forward
- the hurdles that must be cleared before a new DNA profiling methodology can be used is.... A laboratory must demonstrate that it has achieved an error rate of zero with the methodology using samples that mimic those it encounters in casework. A lab needs to develop standard operating procedures and proficiency tests for its analysts. The technique must also be approved by an independent accrediting agency. A lab needs to invest in equipment, validation, the development of standard operating procedures, and training. The technique must also be determined to be admissible after Frye and Daubert hearings. Scientists working in an academic setting must first prepare the methodology for use in crime laboratories by developing test kits. Labs must then independently validate commercially available kits. Private crime laboratories can use new methodologies at any time but state/public crime laboratories must…arrow_forwardWhat are three major limitations of forensic DNA profiling?arrow_forwardWhich of the following criteria do you feel is the most important one to optimize for a DNA profiling methods? Speed Discriminating power Sensitivity Cost False Positive Ratearrow_forward
- What are the function of autoradiography in DNA fingerprinting?arrow_forwardMention any two applications of DNA fingerprinting.arrow_forwardDiscuss the uses of PCR and RT-PCR in human identity (e.g. paternity, forensics or criminal cases) as well as in viral or bacterial DNA/RNA analysis (i.e. Identification of SARS-COV-2 RNA coronavirus using RT-PCR).arrow_forward
- What is DNA fingerprinting? How can it be used in human identification?arrow_forwardWhich of the following ARE part of a typical PCR reaction mixture? DNA ligase dNTPs (mix of nucleoside tri-phosphates) RNA primers made by primase enzyme template DNA, often from cells collected from hair, cheek swab, or blood 2 DNA primers polymerase enzymearrow_forwardExplain the use of forensic DNA fingerprintingor DNA typing ?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Principles Of Radiographic Imaging: An Art And A ...Health & NutritionISBN:9781337711067Author:Richard R. Carlton, Arlene M. Adler, Vesna BalacPublisher:Cengage LearningEssentials Health Info Management Principles/Prac...Health & NutritionISBN:9780357191651Author:BowiePublisher:Cengage
Principles Of Radiographic Imaging: An Art And A ...
Health & Nutrition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Cengage Learning
Essentials Health Info Management Principles/Prac...
Health & Nutrition
ISBN:9780357191651
Author:Bowie
Publisher:Cengage
DNA Use In Forensic Science; Author: DeBacco University;https://www.youtube.com/watch?v=2YIG3lUP-74;License: Standard YouTube License, CC-BY
Analysing forensic evidence | The Laboratory; Author: Wellcome Collection;https://www.youtube.com/watch?v=68Y-OamcTJ8;License: Standard YouTube License, CC-BY