Introduction To Genetic Analysis
12th Edition
ISBN: 9781319114787
Author: Anthony J.F. Griffiths, John Doebley, Catherine Peichel, David A. Wassarman
Publisher: W. H. Freeman
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Chapter 2, Problem 50P
Summary Introduction
To determine: The possible explaination of the presence of low density of ascospores at the edge of the colony plate.
Introduction: The mutation is the change in the
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When two plants belonging to the same genus but different species are crossed, the F1 hybrid is more viable and has more ornate flowers. Unfortunately, this hybrid is sterile and can only be propagated by vegetative cuttings. Explain the sterility of the hybrid and what would have to occur for the sterility of this hybrid to be reversed.
From a cross between e+ f+ g+ and e− f − g− strains ofNeurospora, recombination between these linkedgenes resulted in a few octads containing the followingordered set of spores:e+ f+ g+e+ f+ g+e+ f − g+e+ f − g+e− f − g−e− f − g−e− f − g−e− f − g−a. Where was recombination initiated?b. Did crossing-over occur between genes e and g?Explain.c. Why do you end up with 2 f+ : 6 f − but 4 e+: 4 e−and 4g+: 4g−?d. Could you characterize these unusual octads as MIor MII for any of the three genes involved?Explain.
Neurospora of genotype a + c are crossed withNeurospora of genotype + b +. (Here, + is shorthandfor the wild-type allele.) The following tetrads areobtained (note that the genotype of the four sporepairs in an ascus are listed, rather than listing alleight spores):a + c a b c + + c + b c a b + a + ca + c a b c a + c a b c a b + a b c+ b + + + + + b + + + + + + c + + ++ b + + + + a b + a + + + + c + b +137 141 26 25 2 3a. In how many cells has meiosis occurred to yieldthese data?b. Give the best genetic map to explain these results.Indicate all relevant genetic distances, both betweengenes and between each gene and the centromere.c. Diagram a meiosis that could give rise to oneof the three tetrads in the class at the far right inthe list
Chapter 2 Solutions
Introduction To Genetic Analysis
Ch. 2 - Prob. 1PCh. 2 - Prob. 2PCh. 2 - Prob. 3PCh. 2 - Prob. 4PCh. 2 - Prob. 5PCh. 2 - Prob. 6PCh. 2 - Prob. 7PCh. 2 - Prob. 8PCh. 2 - Prob. 9PCh. 2 - Prob. 10P
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- Imagine that you have done a cross between two strains of yeast, one of which has the genotype A B C and the other a b c, where the letters refer to three closely linked genes in the order given. You examine many tetrads resulting from this cross, and the two tetrads below are found that have atypical scoring patterns. In tetrad I, the spores are A B C, ABC, a B c, and a b c. In tetrad II, the spores are A B C, A b c, a b C, and a b c. Which tetrad or tetrads indicate(s) that gene conversion has occurred AND show(s) that recombination has occurred between genes A and C? O neither tetrads I and II O tetrad II O tetrad I O both tetrads I and IIarrow_forwardYou cross two yeast strains one is an ade auxotroph the other is a pro auxotroph and allow the diploid to sporulate. When you score each spore in the ascus you find the following proportions: 518 PD, 8 NPD, and 225 T. a.) What are the genotypes of each spore in all three types of the tetrads. b) Are these genes linked why or why not? c.) If these genes are unlinked what would you expect the progeny numbers and ratios to be? d.) What is the formula to determine the most accurate distance between these genes? If linked what is the map distance?arrow_forwardA cross was performed between a yeast strain that requires methionine and lysine for growth (met− lys−)and another yeast strain, which is met+ lys+. One hundred asci were dissected, and colonies were grownfrom the four spores in each ascus. Cells from thesecolonies were tested for their ability to grow on petriplates containing either minimal medium (min), min+ lysine (lys), min + methionine (met), or min + lys+ met. The asci could be divided into two groupsbased on this analysis:Group 1: In 89 asci, cells from two of the four spore colonies couldgrow on all four kinds of media, while the other two spore coloniescould grow only on min + lys + met.Group 2: In 11 asci, cells from one of the four spore colonies couldgrow on all four kinds of petri plates. Cells from a second one ofthe four spore colonies could grow only on min + lys plates andon min + lys + met plates. Cells from a third of the four sporecolonies could only grow on min + met plates and on min + lys+ met. Cells from the…arrow_forward
- Chlamydomonas, a eukaryotic green alga, may be sensitive to the antibiotic erythromycin, which inhibits protein synthesis in bacteria. There are two mating types in this alga, mt+ and mt-. If an mt+ cell sensitive to the antibiotic is crossed with an mt- cell that is resistant, all progeny cells are sensitive. The reciprocal cross (mt+ resistant and mt- sensitive) yields all resistant progeny cells. Assuming that the mutation for resistance is in the chloroplast DNA, what can you conclude from the results of these crosses?arrow_forwardA yeast geneticist irradiates haploid cells of a strain that is an adenine-requiring auxotrophic mutant, caused by mutation of the gene ade1. Millions of the irradiated cells are plated on minimal medium, and a small number of cells divide and produce prototrophic colonies. These colonies are crossed individually with a wildtype strain. Two types of results are obtained:(1) prototroph × wild type : progeny all prototrophic(2) prototroph × wild type : progeny 75% prototrophic, 25% adenine-requiring auxotrophsa. Explain the difference between these two types of results.b. Write the genotypes of the prototrophs in each case.c. What progeny phenotypes and ratios do you predict from crossing a prototroph of type 2 by the original ade1auxotroph?arrow_forwardTwo haploid strains of S. cerevisae (yeast) are crossed. One has the genotype ABC and the other abc. Five sets of the resultant tetrads are noted below. In which set did a gene conversion event occur? Look for a 1:3 or 3:1 ratio A) abc, aBc, AbC, aBC a/A is 3 recessive 1dominant, B/b is 2:2, same for C B) abc, abc, ABC, ABC 2:2 for all C) aBc, aBc, AbC, AbC 2:2 for all D) abC, abc, ABc, ABC this one has equal ratio of dominant/recessive 2 dominant C’s, 2 Recessive c, same form A and a and B and b. E) Abc, Abc, aBC, aBCarrow_forward
- In a cross between a white-eyed female (ww) and a red-eyed male (w+Y), nearly all the progeny were either red-eyed females (w+w) or white-eyed males (wY). However, about 1 in every 2000 F1 flies had an "exceptional phenotype" and was either a white-eyed female or red-eyed male. How did Bridges explain this unexpected result? A) Crossing over B) Incomplete cytokinesis C) Incorrect synapsis D) Nondisjunction E) Pseudoautosomal regionarrow_forwardIndividuals of genotype AaBb were mated to individuals of genotype aabb. One thousand offspring were counted, with the following results: 474 Aabb, 480 aaBb, 20 AaBb, and 26 aabb. What type of cross is it? Are these loci linked? What are the two parental classes and the two recombinant classes of offspring? What is the percentage of recombination between these two loci? How many map units apart are they?arrow_forwardA Drosophila strain with two mutated phenotypes was crossed with a wild-type Drosophila strain. The wild-type phenotypes were present in all of the F1 flies. The F1 flies were bred with one another. The F2 generation, on the other hand, did not have the predicted 9:3:3:1 phenotypic ratio. Explain why these outcomes occurred.arrow_forward
- The following results are derived from crosses between Neurospora strain xy and strain ++: Tetrad Class 3 4 ху x+ x+ xy ++ ++ ++ +y +y ху +y 25 ++ 3 124 4 (i) Name the ascus type of each class from 1 to 4 as P, NP or T. (ii) Are genes x and y linked? Explain your answer. (iii) If they are linked, determine the map distance between the two genes. If they are unlinked, provide all the information you can about why you draw this conclusion.arrow_forwardNicotiana glutinosa (2 n = 24) and N. tabacum (2 n = 48) are two closely related plants that can be intercrossed, but the F1 hybrid plants that result are usually sterile. In 1925, Roy Clausen and Thomas Goodspeed crossed N. glutinosa and N. tabacum and obtained one fertile F1 plant . They were able to self-pollinate the flowers of this plant to produce an F2 generation. Surprisingly, the F2 plants were fully fertile and produced viable seeds. When Clausen and Goodspeed examined the chromosomes of the F2 plants, they observed 36 pairs of chromosomes in metaphase I and 36 individual chromosomes in metaphase II. Explain the origin of the F2 plants obtained by Clausen and Goodspeed and the numbers of chromosomes observed.arrow_forwardOn chromosome 4 in Nuerospora, the allele pyr-1 results in a pyrimidine requirement for growth. A cross is made between a pyr-1 and pyr+ strain and the following results were obtained: 26 of a 2:4:2 pattern of pyr-1:pyr+:pyr-1 25 of a 2:4:2 pattern of pyr+:pyr-1:pyr+ 24 of a 2:2:2:2 pattern of pyr-1:pyr+:pyr-1:pyr+ 457 of a 4:4 pattern of pyr-1:pyr+ 27 of a 2:2:2:2 pattern of pyr+:pyr-1:pyr+:pyr-1 452 of a 4:4 pattern of pyr+:pyr-1 What is the distance of the pyr locus to the centromere? Show your work.arrow_forward
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