Biology 2e
Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
bartleby

Concept explainers

bartleby

Videos

Textbook Question
Book Icon
Chapter 17, Problem 1VCQ

Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment?

  1. There will be no colonies on the bacterial
  2. plate.

  • There will be blue colonies only.
  • There will be blue and white colonies.
  • The will be white colonies only.
  • Chapter 17, Problem 1VCQ, Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner

    Expert Solution & Answer
    Check Mark
    Summary Introduction

    Introduction:

    Molecular cloning is a process to reproduce desired fragments of the genome. In cloning the plasmid molecules can be used to provide a folder in which desired genomic fragment is inserted. Then plasmids are introduced into a bacterial host. Plasmids with foreign DNA into them are called recombinant DNA molecules.

    Answer to Problem 1VCQ

    Correct answer:

    The correct answer is option (b)- there will be blue colony only.

    Explanation of Solution

    Explanation for the correct answer:

    Option (b) is given as ‘there will be blue colony only’. In this experiment, when the cloning vector plasmid with degraded foreign DNA is transformed into a bacterial host strain, releases a dye which turns the colony blue. Therefore, option (b) is correct.

    Explanation for incorrect answers:

    Option (a) is given as ‘there will be no colonies on the bacterial plate’. The colonies will be present on the plate. So, it is a wrong answer.

    Option (c) is given as ‘there will be blue and white colonies no colonies’. Both blue and white colonies are only possible when plasmids are with foreign DNA insert and without insert.

    So, it is a wrong answer.

    Option (d) given as ‘there will be white colony only’. White colonies are not possible as plasmids with insert are not capable to express their characters. So, it is a wrong answer.

    Hence, options (a ), (c), and (d) are incorrect.

    Conclusion

    The molecular cloning is a process to reproduce the desired fragments of the genome is reproduced by molecular cloning with the help of plasmid which provides a folder in which desired genomic fragment is inserted. Hence, the correct answer is option (b).

    Want to see more full solutions like this?

    Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!
    Students have asked these similar questions
    Respond to the following in a minimum of 175 words:  How far might science go in the name of genetic research, and at what point should genetic research be regulated? What types of ethical issues might arise from altering the genes of humans, animals, and plants? Provide an example and discuss the advantages and disadvantages of genetic experimentation.
    please help thank you
    How are sharks different than all the other species

    Chapter 17 Solutions

    Biology 2e

    Knowledge Booster
    Background pattern image
    Biology
    Learn more about
    Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
    Similar questions
    SEE MORE QUESTIONS
    Recommended textbooks for you
    Text book image
    Human Heredity: Principles and Issues (MindTap Co...
    Biology
    ISBN:9781305251052
    Author:Michael Cummings
    Publisher:Cengage Learning
    Text book image
    Biology 2e
    Biology
    ISBN:9781947172517
    Author:Matthew Douglas, Jung Choi, Mary Ann Clark
    Publisher:OpenStax
    Text book image
    Biology Today and Tomorrow without Physiology (Mi...
    Biology
    ISBN:9781305117396
    Author:Cecie Starr, Christine Evers, Lisa Starr
    Publisher:Cengage Learning
    Text book image
    Concepts of Biology
    Biology
    ISBN:9781938168116
    Author:Samantha Fowler, Rebecca Roush, James Wise
    Publisher:OpenStax College
    Text book image
    Biology: The Dynamic Science (MindTap Course List)
    Biology
    ISBN:9781305389892
    Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
    Publisher:Cengage Learning
    Text book image
    Biochemistry
    Biochemistry
    ISBN:9781305577206
    Author:Reginald H. Garrett, Charles M. Grisham
    Publisher:Cengage Learning
    Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license