Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Textbook Question
Chapter 12, Problem 19P
14CO2 was bubbled through a suspension of liver cells that was undergoing gluconeogenesis from lactate to glucose. Which carbons in the glucose molecule would become radioactive?
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14CO2 was bubbled through a suspension of liver cells that was undergoinggluconeogenesis from lactate to glucose. Which carbons in the glucose molecule would become radioactive?
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Chapter 12 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 12 - Prob. 1PCh. 12 - Methanol is highly toxic, not because of its own...Ch. 12 - Prob. 3PCh. 12 - Prob. 4PCh. 12 - Prob. 5PCh. 12 - Write balanced chemical equations for each of the...Ch. 12 - Prob. 7PCh. 12 - Prob. 8PCh. 12 - Prob. 9PCh. 12 - Prob. 10P
Ch. 12 - Some anaerobic bacteria use alternative pathways...Ch. 12 - Write a pathway leading from glucose to lactose in...Ch. 12 - Prob. 13PCh. 12 - Prob. 14PCh. 12 - Prob. 15PCh. 12 - How many ATP equivalents are consumed in the...Ch. 12 - Prob. 17PCh. 12 - Avidin is a protein that binds extremely tightly...Ch. 12 - 14CO2 was bubbled through a suspension of liver...Ch. 12 - Prob. 20PCh. 12 - Prob. 21PCh. 12 - Prob. 22PCh. 12 - Prob. 23PCh. 12 - 24. Glycogen synthesis and breakdown are regulated...Ch. 12 - Prob. 25PCh. 12 - Prob. 26PCh. 12 - Prob. 27PCh. 12 - Prob. 28PCh. 12 - 29 [1-14C] Ribose-5-phosphate is incubated with a...Ch. 12 - Prob. 30PCh. 12 - Prob. 31PCh. 12 - Prob. 32PCh. 12 - Prob. 33P
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- The four-carbon sugar erythrose-4-phosphate is the starting material in the synthesis of many compounds. Name two important examples.arrow_forwardIdentify each reaction catalyzed by (a) a nucleotidase; (b) a phosphorylase;(c) a phosphoribosyltransferase.arrow_forward"CO, was bubbled through a suspension of liver cells that was undergo- ing gluconeogenesis from lactate to glucose. Which carbons in the glucose molecule would become radioactive?arrow_forward
- (a) Draw the structure of the high-energy nucleoside triphosphate GTP. (b) Draw the structure of the hydrolysis product formed when one phosphate is removed.arrow_forwardOf course, one cannot stick a piece of tape to each carbon atom in the actual glucosemolecule. But one can do the next best thing: use radioisotopes to trace themovement of various atoms. Specifically, because carbon dioxide is a gas, usingradioactive C-14 allows for the easy detection of the 14CO2 that is given off.Suppose you introduce one glucose molecule that has had all of its carbons radioactivelylabeled. Suppose further that each step in glycolysis and in the citric acid cycle takes oneminute to complete (so glycolysis, for instance, takes ten minutes to complete, theconversion to acetyl CoA takes one minute, and the citric acid cycle takes eight minutes).Draw a plot of time (x-axis) versus radioactivity detected by a gas sensor (y-axis). Labelthe x-axis with enough time increments so it is clear at what minute the radioactivitywas detected. Use differing peak heights to give an indication of the amount ofradioactivity detected at that point.arrow_forwardIt is important to note that galactose is an important component of glyco- proteins. What is the effect on glycoprotein synthesis in case of galactosemic patients who are not given galactose in their diet? Explain this observation.arrow_forward
- Why "cellulose is composed of a long, branced chain of B-glucose subunits" is false?arrow_forwardA glycogen polymer and an amylopectin polymer, each containing 100 monosaccharide subunits, are cleaved completely by an enzyme. How many individual glucose molecules are created during this process?arrow_forwardOnly section 6-31arrow_forward
- Human insulin is prodụced as a recombinant protein in the yeast Saccharomyces cerevisiae The protein is extracted and purified for the treatment of diabetes -a $32 billion industry! For efficacy, insulin must be modified by the addition of oligosaccharyl (sugar) groups If you were a scientist working on this project, how would you isolate the glycosylated insulin from the un- glycosylated forms?arrow_forwardPlease, please I do not understand this question for Biochemistry. Can you help me? Steps leading to the correct answer would be helpful!arrow_forwardA solution of [U 14C] glucose-1-phosphate (specific activity = 16,000 cpm/mmole) was incubated with glycogen and glycogen phosphorylase, an enzyme which adds glucose units on to glycogen. Radioactivity was incorporated into the glycogen primer at a rate = 2550 cpm/min. The rate of the enzymatic reaction in units of mmole glucose incorporated per minute is: (a) 0.016 mmol/min (b) 0.57 mmol/min (c) 0.16 mmol/min (d) 5.7 mmol/minarrow_forward
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