(a) Interpretation:
To determine the steady state kinetic analysis of the muscle isozyme by lactate which is present or not by the following inhibited/uninhibited enzymes:
Introduction:
Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.
(b) Interpretation:
The reciprocal of Vmax for the uninhibited enzyme.
Introduction:
Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.
(c) Interpretation:
The line representing the data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate.
Introduction:
Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.
(d) Interpretation:
The line representing data obtained in the presence of lactate acting as a noncompetitive inhibitor with respect to the variable substrate.
Introduction:
Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.
(e) Interpretation:
The reciprocal of KM in the presence of lactate acting as a competitive inhibitor.
Introduction:
Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.
(f) Interpretation:
The most appropriate NADH concentration to use when determining KM for pyruvate.
Introduction:
Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.
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Biochemistry: Concepts and Connections (2nd Edition)
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