(a) Interpretation: To determine the steady state kinetic analysis of the muscle isozyme by lactate which is present or not by the following inhibited/uninhibited enzymes: Introduction: Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

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Answer 1

Question

Chapter 12, Problem 15P

Interpretation Introduction

(a) Interpretation:

To determine the steady state kinetic analysis of the muscle isozyme by lactate which is present or not by the following inhibited/uninhibited enzymes:

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(b) Interpretation:

The reciprocal of Vmax for the uninhibited enzyme.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(c) Interpretation:

The line representing the data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(d) Interpretation:

The line representing data obtained in the presence of lactate acting as a noncompetitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(e) Interpretation:

The reciprocal of K_M in the presence of lactate acting as a competitive inhibitor.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(f) Interpretation:

The most appropriate NADH concentration to use when determining K_M for pyruvate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

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Answer 2

Question

Chapter 12, Problem 15P

Interpretation Introduction

(a) Interpretation:

To determine the steady state kinetic analysis of the muscle isozyme by lactate which is present or not by the following inhibited/uninhibited enzymes:

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(b) Interpretation:

The reciprocal of Vmax for the uninhibited enzyme.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(c) Interpretation:

The line representing the data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(d) Interpretation:

The line representing data obtained in the presence of lactate acting as a noncompetitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(e) Interpretation:

The reciprocal of K_M in the presence of lactate acting as a competitive inhibitor.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(f) Interpretation:

The most appropriate NADH concentration to use when determining K_M for pyruvate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

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Answer 3

Question

Chapter 12, Problem 15P

Interpretation Introduction

(a) Interpretation:

To determine the steady state kinetic analysis of the muscle isozyme by lactate which is present or not by the following inhibited/uninhibited enzymes:

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(b) Interpretation:

The reciprocal of Vmax for the uninhibited enzyme.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(c) Interpretation:

The line representing the data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(d) Interpretation:

The line representing data obtained in the presence of lactate acting as a noncompetitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(e) Interpretation:

The reciprocal of K_M in the presence of lactate acting as a competitive inhibitor.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(f) Interpretation:

The most appropriate NADH concentration to use when determining K_M for pyruvate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Expert Solution & Answer

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Answer 4

Question

Chapter 12, Problem 15P

Interpretation Introduction

(a) Interpretation:

To determine the steady state kinetic analysis of the muscle isozyme by lactate which is present or not by the following inhibited/uninhibited enzymes:

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(b) Interpretation:

The reciprocal of Vmax for the uninhibited enzyme.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(c) Interpretation:

The line representing the data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(d) Interpretation:

The line representing data obtained in the presence of lactate acting as a noncompetitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(e) Interpretation:

The reciprocal of K_M in the presence of lactate acting as a competitive inhibitor.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(f) Interpretation:

The most appropriate NADH concentration to use when determining K_M for pyruvate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 12, Problem 15P

Interpretation Introduction

(a) Interpretation:

To determine the steady state kinetic analysis of the muscle isozyme by lactate which is present or not by the following inhibited/uninhibited enzymes:

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(b) Interpretation:

The reciprocal of Vmax for the uninhibited enzyme.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(c) Interpretation:

The line representing the data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(d) Interpretation:

The line representing data obtained in the presence of lactate acting as a noncompetitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(e) Interpretation:

The reciprocal of K_M in the presence of lactate acting as a competitive inhibitor.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(f) Interpretation:

The most appropriate NADH concentration to use when determining K_M for pyruvate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 6

Chapter 12, Problem 15P

Interpretation Introduction

(a) Interpretation:

To determine the steady state kinetic analysis of the muscle isozyme by lactate which is present or not by the following inhibited/uninhibited enzymes:

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 7

Chapter 12, Problem 15P

Interpretation Introduction

(a) Interpretation:

To determine the steady state kinetic analysis of the muscle isozyme by lactate which is present or not by the following inhibited/uninhibited enzymes:

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 8

Interpretation Introduction

(b) Interpretation:

The reciprocal of Vmax for the uninhibited enzyme.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 9

Interpretation Introduction

(b) Interpretation:

The reciprocal of Vmax for the uninhibited enzyme.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 10

Interpretation Introduction

(c) Interpretation:

The line representing the data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 11

Interpretation Introduction

(c) Interpretation:

The line representing the data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 12

Interpretation Introduction

(d) Interpretation:

The line representing data obtained in the presence of lactate acting as a noncompetitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 13

Interpretation Introduction

(d) Interpretation:

The line representing data obtained in the presence of lactate acting as a noncompetitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 14

Interpretation Introduction

(e) Interpretation:

The reciprocal of K_M in the presence of lactate acting as a competitive inhibitor.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 15

Interpretation Introduction

(e) Interpretation:

The reciprocal of K_M in the presence of lactate acting as a competitive inhibitor.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 16

Interpretation Introduction

(f) Interpretation:

The most appropriate NADH concentration to use when determining K_M for pyruvate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 17

Interpretation Introduction

(f) Interpretation:

The most appropriate NADH concentration to use when determining K_M for pyruvate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Answer 18

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Answer 19

Expert Solution & Answer

Answer 20

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Answer 21

Ch. 12 - Prob. 1P Ch. 12 - Methanol is highly toxic, not because of its own...Ch. 12 - Prob. 3P Ch. 12 - Prob. 4P Ch. 12 - Prob. 5P Ch. 12 - Write balanced chemical equations for each of the...Ch. 12 - Prob. 7P Ch. 12 - Prob. 8P Ch. 12 - Prob. 9P Ch. 12 - Prob. 10P

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