Suppose the data below are obtained for an enzyme catalyzed reaction with and without the inhibitor I. (s)( mM) 0.2 0.4 0.8 1.0 2.0 4.0 V without i (mM/min) 5.0 7.5 10.0 10.7 12.5 13.6 V with I (mM/min) 3.0 5.0 7.5 8.3 10.7 12.5 Make a Lineweaver Burke plot for this data using graph paper or a spreadsheet Calculate KM and Vmax without inhibitor. What type of inhibition is observed? show graph and work 2. Give the Lineweaver Burk equation and define all the parameters. 3. When substrate concentration is much greater than Km, the rate of catalysis is almost equal to a. kcat b. none of these c. all of these d. Kd e. Vmax

Suppose the data below are obtained for an enzyme catalyzed reaction with and without the inhibitor I. (s)( mM) 0.2 0.4 0.8 1.0 2.0 4.0 V without i (mM/min) 5.0 7.5 10.0 10.7 12.5 13.6 V with I (mM/min) 3.0 5.0 7.5 8.3 10.7 12.5 Make a Lineweaver Burke plot for this data using graph paper or a spreadsheet Calculate KM and Vmax without inhibitor. What type of inhibition is observed? show graph and work 2. Give the Lineweaver Burk equation and define all the parameters. 3. When substrate concentration is much greater than Km, the rate of catalysis is almost equal to a. kcat b. none of these c. all of these d. Kd e. Vmax

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter6: Oral Medication Labels And Dosage Calculation

Section: Chapter Questions

Problem 7.2P

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