URGENT Cisplatin si a compound, hypothesized to target mammalian Cytochrome C, localized ni the mitochondrial electron transport chain. You have a culture of hepatocellular HepG2 cells, that you have treated with Cisplatin at 2 uM for 3 hours.Based on what you have learned from fluorescent microscopy in this tutorial, design an experimentto investigate whether this compound affects the mitochondrial ETC, in HepG2 cells. You have access to all the fluorophores, fluorescent probes and fluorescent proteins shown in the table below, alongwith technologies to label antibodies and create fluorescent protein expressing cell lines. In your experimental planning include:1) 2) 3)4) 5)Experimental justification and principle.How you will determine mitochondrial function with your selected fluorophores.Complementary fluorophores to perform mitochondrial colocalization. Draw an image of your excitation and emission spectra.The positive and negative controls in your experiment. The expected results of your experiment.
URGENT
Cisplatin si a compound, hypothesized to target mammalian Cytochrome C, localized ni the mitochondrial electron transport chain. You have a culture of hepatocellular HepG2 cells, that you have treated with Cisplatin at 2 uM for 3 hours.
Based on what you have learned from fluorescent microscopy in this tutorial, design an experiment
to investigate whether this compound affects the mitochondrial ETC, in HepG2 cells. You have access to all the fluorophores, fluorescent probes and fluorescent proteins shown in the table below, along
with technologies to label antibodies and create fluorescent protein expressing cell lines. In your experimental planning include:
1) 2) 3)
4) 5)
Experimental justification and principle.
How you will determine mitochondrial function with your selected fluorophores.
Complementary fluorophores to perform mitochondrial colocalization. Draw an image of your excitation and emission spectra.
The positive and negative controls in your experiment. The expected results of your experiment.
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