Your identify a number of mutants and start to characterize the problems in their mismatch repair mechanisms. Based on the following initial results of your experiments, predict which mismatch repair protein(s) is mutated in each mutant. (Your experiments tells you what mismatches are being left unfixed.) Mutant 1: Still contains mismatches where G binds to T and T binds to G Mutant 2: Roughly one half of all mismatches remain. Mutant 3: All mutations remain, but you find that no mismatch repair proteins are non-functional. Mutant 4: All mutations remain, and you find increased MutL-MutS complexes bound to the
Bacterial Genomics
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Transformation Experiment in Bacteria
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Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Your identify a number of mutants and start to characterize the problems in their mismatch repair mechanisms. Based on the following initial results of your experiments, predict which mismatch repair protein(s) is mutated in each mutant. (Your experiments tells you what mismatches are being left unfixed.)
Mutant 1: Still contains mismatches where G binds to T and T
binds to G
Mutant 2: Roughly one half of all mismatches remain.
Mutant 3: All mutations remain, but you find that no mismatch repair proteins are non-functional.
Mutant 4: All mutations remain, and you find increased MutL-MutS
complexes bound to the DNA.
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