Homework Help is Here – Start Your Trial Now!
Science
Biology
The protein AKT/PKB is a critical regulator of essential cellular processes, and dysregulation of AKT has been implicated in many diseases, including cancer. In the present study, we wanted to test if levels of protein AKT changed in in EGF-stimulated epithelial, human breast cancer cell line when treated with varying concentrations of a drug Chondramide (ChB; 30 nM or 100 nM) for different lengths of time (1 or 24 h). Cellular lysate was prepared for each experimental condition, and an equal concentration of protein was loaded into a single lane of the SDS-polyacrylamide gel for each sample. We used western blot to measure the total levels of AKT and the fraction of Akt that is phosphorylated on the serine residue at position 473 (p-AktS473) using a phosphorylation specific antibody. GAPDH was used a loading control. EGF 30 nM ChB 100 nM ChB P-Akers total Akt GAPDH 0 0 + 0 0 1h 0 24h 0 0 0 1h 24h Why do you think the researchers included GAPDH in the figure? ABCD A. B. C. D. Control for cell condition Control for unwanted proteins Positive control for Antibody for AKT Control for amount of protein loaded Indicate for the following statements is they are supported/not supported by the data in the figure. Statement 1. Phosphorylation of AKT (S473) increases upon EGF stimulation Statement 2. The total amount of AKT in the cell reduces upon EGF stimulation_ Statement 3. ChB treatment (both concentration and length of time) increases the amount of phosphorylated AKT.

The protein AKT/PKB is a critical regulator of essential cellular processes, and dysregulation of AKT has been implicated in many diseases, including cancer. In the present study, we wanted to test if levels of protein AKT changed in in EGF-stimulated epithelial, human breast cancer cell line when treated with varying concentrations of a drug Chondramide (ChB; 30 nM or 100 nM) for different lengths of time (1 or 24 h). Cellular lysate was prepared for each experimental condition, and an equal concentration of protein was loaded into a single lane of the SDS-polyacrylamide gel for each sample. We used western blot to measure the total levels of AKT and the fraction of Akt that is phosphorylated on the serine residue at position 473 (p-AktS473) using a phosphorylation specific antibody. GAPDH was used a loading control. EGF 30 nM ChB 100 nM ChB P-Akers total Akt GAPDH 0 0 + 0 0 1h 0 24h 0 0 0 1h 24h Why do you think the researchers included GAPDH in the figure? ABCD A. B. C. D. Control for cell condition Control for unwanted proteins Positive control for Antibody for AKT Control for amount of protein loaded Indicate for the following statements is they are supported/not supported by the data in the figure. Statement 1. Phosphorylation of AKT (S473) increases upon EGF stimulation Statement 2. The total amount of AKT in the cell reduces upon EGF stimulation_ Statement 3. ChB treatment (both concentration and length of time) increases the amount of phosphorylated AKT.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
The protein AKT/PKB is a critical regulator of essential cellular processes, and dysregulation of AKT has been implicated in many diseases, including cancer. In the present study, we wanted to test if levels of protein AKT changed in in EGF-stimulated epithelial, human breast cancer cell line when treated with varying concentrations of a drug Chondramide (ChB; 30 nM or 100 nM) for different lengths of time (1 or 24 h). Cellular lysate was prepared for each experimental condition, and an equal concentration of protein was loaded into a single lane of the SDS-polyacrylamide gel for each sample. We used western blot to measure the total levels of AKT and the fraction of Akt that is phosphorylated on the serine residue at position 473 (p-AktS473) using a phosphorylation specific antibody. GAPDH was used a loading control. EGF 30 nM ChB 100 nM ChB P-Ak47 total Akt GAPDH 0 0 + 0 0 0 0 0 1h 24h 1h 24h 0 Why do you think the researchers included GAPDH in the figure? A. B. C. D. Control for cell condition Control for unwanted proteins Positive control for Antibody for AKT Control for amount of protein loaded Indicate for the following statements is they are supported/not supported by the data in the figure. Statement 1. Phosphorylation of AKT (S473) increases upon EGF stimulation Statement 2. The total amount of AKT in the cell reduces upon EGF stimulation_ Statement 3. ChB treatment (both concentration and length of time) increases the amount of phosphorylated AKT.
Transcribed Image Text:The protein AKT/PKB is a critical regulator of essential cellular processes, and dysregulation of AKT has been implicated in many diseases, including cancer. In the present study, we wanted to test if levels of protein AKT changed in in EGF-stimulated epithelial, human breast cancer cell line when treated with varying concentrations of a drug Chondramide (ChB; 30 nM or 100 nM) for different lengths of time (1 or 24 h). Cellular lysate was prepared for each experimental condition, and an equal concentration of protein was loaded into a single lane of the SDS-polyacrylamide gel for each sample. We used western blot to measure the total levels of AKT and the fraction of Akt that is phosphorylated on the serine residue at position 473 (p-AktS473) using a phosphorylation specific antibody. GAPDH was used a loading control. EGF 30 nM ChB 100 nM ChB P-Ak47 total Akt GAPDH 0 0 + 0 0 0 0 0 1h 24h 1h 24h 0 Why do you think the researchers included GAPDH in the figure? A. B. C. D. Control for cell condition Control for unwanted proteins Positive control for Antibody for AKT Control for amount of protein loaded Indicate for the following statements is they are supported/not supported by the data in the figure. Statement 1. Phosphorylation of AKT (S473) increases upon EGF stimulation Statement 2. The total amount of AKT in the cell reduces upon EGF stimulation_ Statement 3. ChB treatment (both concentration and length of time) increases the amount of phosphorylated AKT.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Mutation
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS